دورية أكاديمية
Targeted Imaging of Endometriosis and Image-Guided Resection of Lesions Using Gonadotropin-Releasing Hormone Analogue-Modified Indocyanine Green
| العنوان: | Targeted Imaging of Endometriosis and Image-Guided Resection of Lesions Using Gonadotropin-Releasing Hormone Analogue-Modified Indocyanine Green |
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| المؤلفون: | Jing Peng, Qiyu Liu, Tao Pu, Mingxing Zhang, Meng Zhang, Ming Du, Guiling Li, Xiaoyan Zhang, Congjian Xu |
| المصدر: | Molecular Imaging, Vol 2023 (2023) |
| بيانات النشر: | SAGE Publications, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biology (General) LCC:Medical technology |
| مصطلحات موضوعية: | Biology (General), QH301-705.5, Medical technology, R855-855.5 |
| الوصف: | Objective. In this study, we utilized gonadotropin-releasing hormone analogue-modified indocyanine green (GnRHa-ICG) to improve the accuracy of intraoperative recognition and resection of endometriotic lesions. Methods. Gonadotropin-releasing hormone receptor (GnRHR) expression was detected in endometriosis tissues and cell lines via immunohistochemistry and western blotting. The in vitro binding capacities of GnRHa, GnRHa-ICG, and ICG were determined using fluorescence microscopy and flow cytometry. In vivo imaging was performed in mouse models of endometriosis using a near-infrared fluorescence (NIRF) imaging system and fluorescence navigation system. The ex vivo binding capacity was determined using confocal fluorescence microscopy. Results. GnRHa-ICG exhibited a significantly stronger binding capacity to endometriotic cells and tissues than ICG. In mice with endometriosis, GnRHa-ICG specifically imaged endometriotic tissues (EMTs) after intraperitoneal administration, whereas ICG exhibited signals in the intestine. GnRHa-ICG showed the highest fluorescence signals in the EMTs at 2 h and a good signal-to-noise ratio at 48 h postadministration. Compared with traditional surgery under white light, targeted NIRF imaging-guided surgery completely resected endometriotic lesions with a sensitivity of 97.3% and specificity of 77.8%. No obvious toxicity was observed in routine blood tests, serum biochemicals, or histopathology in mice. Conclusions. GnRHa-ICG specifically recognized and localized endometriotic lesions and guided complete resection of lesions with high accuracy. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1536-0121 |
| العلاقة: | https://doaj.org/toc/1536-0121Test |
| DOI: | 10.1155/2023/6674054 |
| الوصول الحر: | https://doaj.org/article/c7289c43fce849e4bacafaae8a65c0d7Test |
| رقم الانضمام: | edsdoj.7289c43fce849e4bacafaae8a65c0d7 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 15360121 |
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| DOI: | 10.1155/2023/6674054 |