دورية أكاديمية
Novel Doxorubicin Derivatives: Synthesis and Cytotoxicity Study in 2D and 3D in Vitro Models
| العنوان: | Novel Doxorubicin Derivatives: Synthesis and Cytotoxicity Study in 2D and 3D in Vitro Models |
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| المؤلفون: | Roman Akasov, Maria Drozdova, Daria Zaytseva-Zotova, Maria Leko, Pavel Chelushkin, Annie Marc, Isabelle Chevalot, Sergey Burov, Natalia Klyachko, Thierry Vandamme, Elena Markvicheva |
| المصدر: | Advanced Pharmaceutical Bulletin, Vol 7, Iss 4, Pp 593-601 (2017) |
| بيانات النشر: | Tabriz University of Medical Sciences, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Therapeutics. Pharmacology |
| مصطلحات موضوعية: | Aantitumor drug screening assays, Microencapsulation, Multicellular spheroids, Multiple drug resistance, Serum albumin, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | Purpose: Multidrug resistance (MDR) of tumors to chemotherapeutics often leads to failure of cancer treatment. The aim of the study was to prepare novel MDR-overcoming chemotherapeutics based on doxorubicin (DOX) derivatives and to evaluate their efficacy in 2D and 3D in vitro models. Methods: To overcome MDR, we synthesized five DOX derivatives, and then obtained non-covalent complexes with human serum albumin (HSA). Drug efficacy was evaluated for two tumor cell lines, namely human breast adenocarcinoma MCF-7 cells and DOX resistant MCF-7/ADR cells. Additionally, MCF-7 cells were entrapped in alginate-oligochitosan microcapsules, and generated tumor spheroids were used as a 3D in vitro model to study cytotoxicity of the DOX derivatives. Results: Due to 3D structure, the tumor spheroids were more resistant to chemotherapy compared to monolayer culture. DOX covalently attached to palmitic acid through hydrazone linkage (DOX-N2H-Palm conjugate) was found to be the most promising derivative. Its accumulation levels within MCF-7/ADR cells was 4- and 10-fold higher than those of native DOX when the conjugate was added to cultivation medium without serum and to medium supplemented with 10% fetal bovine serum, respectively. Non-covalent complex of the conjugate with HSA was found to reduce the IC50 value from 32.9 µM (for free DOX-N2H-Palm) to 16.8 µM (for HSA-DOX-N2H-Palm) after 72 h incubation with MCF-7/ADR cells. Conclusion: Palm-N2H-DOX conjugate was found to be the most promising DOX derivative in this research. The formation of non-covalent complex of Palm-N2H-DOX conjugate with HSA allowed improving its anti-proliferative activity against both MCF-7 and MCF-7/ADR cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2228-5881 2251-7308 |
| العلاقة: | http://apb.tbzmed.ac.ir/PDF/apb-7-593.pdfTest; https://doaj.org/toc/2228-5881Test; https://doaj.org/toc/2251-7308Test |
| DOI: | 10.15171/apb.2017.071 |
| الوصول الحر: | https://doaj.org/article/705053c8e34c4bd49f38e564ed7f4eb6Test |
| رقم الانضمام: | edsdoj.705053c8e34c4bd49f38e564ed7f4eb6 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22285881 22517308 |
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| DOI: | 10.15171/apb.2017.071 |