دورية أكاديمية
Comparison of Three Expanded-Spectrum Cephalosporin Hydrolysis Assays and the NG-Test CTX-M Multi Assay That Detects All CTX-M-Like Enzymes
| العنوان: | Comparison of Three Expanded-Spectrum Cephalosporin Hydrolysis Assays and the NG-Test CTX-M Multi Assay That Detects All CTX-M-Like Enzymes |
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| المؤلفون: | Camille Gonzalez, Christian Moguet, Arnaud Chalin, Saoussen Oueslati, Laurent Dortet, Stéphanie Simon, Hervé Volland, Thierry Naas |
| المصدر: | Diagnostics, Vol 12, Iss 1, p 197 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine (General) |
| مصطلحات موضوعية: | biochemical assay, CTX-M, ESBL detection, expanded-spectrum cephalosporin hydrolysis, lateral flow immunoassay, LFIA, Medicine (General), R5-920 |
| الوصف: | Rapid detection of expanded-spectrum cephalosporins (ESC) hydrolysing enzymes is crucial to implement infection control measures and antibiotic stewardship. Here, we have evaluated three biochemical ESC hydrolysis assays (ESBL NDP test, β-LACTA™ test, LFIA-CTX assay) and the NG-Test® CTX-M MULTI that detects CTX-M enzymes, on 93 well-characterized Gram-negative isolates, including 60 Enterobacterales, 21 Pseudomonas spp. and 12 Acinetobacter spp. The performances were good for all three hydrolysis assays, with the LFIA-CTX being slightly more sensitive and specific on the tested panel of isolates especially with Enterobacterales, without ambiguous results. This study showed that LFIA-CTX may be used for the detection of ESC hydrolysis as a competitive alternative to already available assays (β-LACTA™ test and ESBL NDP test) without any specific equipment and reduced hands-on-time. The lateral flow immunoassay NG-Test® CTX-M MULTI has proven to be a useful, easy, rapid, and reliable confirmatory test in Enterobacterales for detection of CTX-M-type ESBLs, which account for most of the resistance mechanisms leading to ESC resistance in Enterobacterales, but it misses rare ESC hydrolysing β-lactamases (AmpC, minor ESBLs, and carbapenemases). Combining it with the LFIA-CTX assay would yield an assay detecting the most frequently-encountered ESBLs (CTX-M-like β-lactamases) together with ESC hydrolysis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 12010197 2075-4418 |
| العلاقة: | https://www.mdpi.com/2075-4418/12/1/197Test; https://doaj.org/toc/2075-4418Test |
| DOI: | 10.3390/diagnostics12010197 |
| الوصول الحر: | https://doaj.org/article/703f8e31c692453e965c9506bcd5f0e1Test |
| رقم الانضمام: | edsdoj.703f8e31c692453e965c9506bcd5f0e1 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 12010197 20754418 |
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| DOI: | 10.3390/diagnostics12010197 |