دورية أكاديمية
Inhibition of the NLRP3/IL‐1β axis protects against sepsis‐induced cardiomyopathy
| العنوان: | Inhibition of the NLRP3/IL‐1β axis protects against sepsis‐induced cardiomyopathy |
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| المؤلفون: | Katharina Busch, Melanie Kny, Nora Huang, Tilman E. Klassert, Magdalena Stock, Alexander Hahn, Sebastian Graeger, Mihail Todiras, Sibylle Schmidt, Bishwas Chamling, Michael Willenbrock, Stefan Groß, Doreen Biedenweg, Arnd Heuser, Claus Scheidereit, Christian Butter, Stephan B. Felix, Oliver Otto, Friedrich C. Luft, Hortense Slevogt, Jens Fielitz |
| المصدر: | Journal of Cachexia, Sarcopenia and Muscle, Vol 12, Iss 6, Pp 1653-1668 (2021) |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Diseases of the musculoskeletal system LCC:Human anatomy |
| مصطلحات موضوعية: | NLR family, pyrin domain‐containing 3 protein, Interleukin‐1 beta, Sepsis, Heart failure, Diseases of the musculoskeletal system, RC925-935, Human anatomy, QM1-695 |
| الوصف: | Abstract Background Septic cardiomyopathy worsens the prognosis of critically ill patients. Clinical data suggest that interleukin‐1β (IL‐1β), activated by the NLRP3 inflammasome, compromises cardiac function. Whether or not deleting Nlrp3 would prevent cardiac atrophy and improve diastolic cardiac function in sepsis was unclear. Here, we investigated the role of NLRP3/IL‐1β in sepsis‐induced cardiomyopathy and cardiac atrophy. Methods Male Nlrp3 knockout (KO) and wild‐type (WT) mice were exposed to polymicrobial sepsis by caecal ligation and puncture (CLP) surgery (KO, n = 27; WT, n = 33) to induce septic cardiomyopathy. Sham‐treated mice served as controls (KO, n = 11; WT, n = 16). Heart weights and morphology, echocardiography and analyses of gene and protein expression were used to evaluate septic cardiomyopathy and cardiac atrophy. IL‐1β effects on primary and immortalized cardiomyocytes were investigated by morphological and molecular analyses. IonOptix and real‐time deformability cytometry (RT‐DC) analysis were used to investigate functional and mechanical effects of IL‐1β on cardiomyocytes. Results Heart morphology and echocardiography revealed preserved systolic (stroke volume: WT sham vs. WT CLP: 33.1 ± 7.2 μL vs. 24.6 ± 8.7 μL, P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2190-6009 2190-5991 |
| العلاقة: | https://doaj.org/toc/2190-5991Test; https://doaj.org/toc/2190-6009Test |
| DOI: | 10.1002/jcsm.12763 |
| الوصول الحر: | https://doaj.org/article/c6eda1ee71e947879615bcafea821786Test |
| رقم الانضمام: | edsdoj.6eda1ee71e947879615bcafea821786 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 21906009 21905991 |
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| DOI: | 10.1002/jcsm.12763 |