دورية أكاديمية
The Effect of Activated FXIII, a Transglutaminase, on Vascular Smooth Muscle Cells
| العنوان: | The Effect of Activated FXIII, a Transglutaminase, on Vascular Smooth Muscle Cells |
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| المؤلفون: | Réka Bogáti, Éva Katona, Amir H. Shemirani, Enikő Balogh, Helga Bárdos, Viktória Jeney, László Muszbek |
| المصدر: | International Journal of Molecular Sciences, Vol 23, Iss 10, p 5845 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | factor XIII, vascular smooth muscle cell, cell proliferation, collagen secretion, thrombospondin-1, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Plasma factor XIII (pFXIII) is a heterotetramer of FXIII-A and FXIII-B subunits. The cellular form (cFXIII), a dimer of FXIII-A, is present in a number of cell types. Activated FXIII (FXIIIa), a transglutaminase, plays an important role in clot stabilization, wound healing, angiogenesis and maintenance of pregnancy. It has a direct effect on vascular endothelial cells and fibroblasts, which have been implicated in the development of atherosclerotic plaques. Our aim was to explore the effect of FXIIIa on human aortic smooth muscle cells (HAoSMCs), another major cell type in the atherosclerotic plaque. Osteoblastic transformation induced by Pi and Ca2+ failed to elicit the expression of cFXIII in HAoSMCs. EZ4U, CCK-8 and CytoSelect Wound Healing assays were used to investigate cell proliferation and migration. The Sircol Collagen Assay Kit was used to monitor collagen secretion. Thrombospondin-1 (TSP-1) levels were measured by ELISA. Cell-associated TSP-1 was detected by the immunofluorescence technique. The TSP-1 mRNA level was estimated by RT-qPCR. Activated recombinant cFXIII (rFXIIIa) increased cell proliferation and collagen secretion. In parallel, a 67% decrease in TSP-1 concentration in the medium and a 2.5-fold increase in cells were observed. TSP-1 mRNA did not change significantly. These effects of FXIIIa might contribute to the pathogenesis of atherosclerotic plaques. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| العلاقة: | https://www.mdpi.com/1422-0067/23/10/5845Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test |
| DOI: | 10.3390/ijms23105845 |
| الوصول الحر: | https://doaj.org/article/6d9dc0d3cd7744d7a205f0de512af863Test |
| رقم الانضمام: | edsdoj.6d9dc0d3cd7744d7a205f0de512af863 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms23105845 |