دورية أكاديمية
Preparation of carrierized cellulase Cellulase@MIL-88B(Fe) and its enzymatic properties
| العنوان: | Preparation of carrierized cellulase Cellulase@MIL-88B(Fe) and its enzymatic properties |
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| المؤلفون: | Erhong Zhang, Meng Wang, Xiaoliang Pan, Xinfeng Wang, Wenju Zhang |
| المصدر: | Green Chemistry Letters and Reviews, Vol 15, Iss 3, Pp 627-637 (2022) |
| بيانات النشر: | Taylor & Francis Group, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Science LCC:Chemistry |
| مصطلحات موضوعية: | Cellulase, immobilization, rigidity, stability, enzymatic hydrolysis, Science, Chemistry, QD1-999 |
| الوصف: | In this study, cellulase was modified by immobilization technique using MIL-88B(Fe) as the carrier, and the immobilization temperature, time, cellulase concentration and pH of 30°C, 6 h, 1.2 mg/mL and 5.0, respectively, were investigated as the optimal preparation conditions. Characterization analysis using FT-IR, XRD and TGA revealed that the successful immobilization of cellulase on MIL-88B(Fe) did not disrupt the integrity of the carrier lattice structure. Compared with the free enzyme, Cellulase@MIL-88B(Fe) maintained 78% and 85.21% of its activity after 10 cycles and 49 d, respectively, and its t1/2 and Ed values were higher than those of the free enzyme, and it was able to maintain a higher activity over a wider pH and temperature range, indicating its higher thermal stability and heat-loss activity. In addition, the Km of Cellulase@MIL-88B(Fe) (1.46 mg/mL) was slightly higher than that of free cellulase (1.41 mg/mL), and Cellulase@MIL-88B(Fe) was able to be applied in the actual enzymatic digestion of corn cobs. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 17518253 1751-7192 1751-8253 |
| العلاقة: | https://doaj.org/toc/1751-8253Test; https://doaj.org/toc/1751-7192Test |
| DOI: | 10.1080/17518253.2022.2124890 |
| الوصول الحر: | https://doaj.org/article/6cdd74554e2a42f683c9da3820f6d691Test |
| رقم الانضمام: | edsdoj.6cdd74554e2a42f683c9da3820f6d691 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 17518253 17517192 |
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| DOI: | 10.1080/17518253.2022.2124890 |