دورية أكاديمية
Long non‐coding RNA MALAT1 promotes Th2 differentiation by regulating microRNA‐135b‐5p/GATA‐3 axis in children with allergic rhinitis
| العنوان: | Long non‐coding RNA MALAT1 promotes Th2 differentiation by regulating microRNA‐135b‐5p/GATA‐3 axis in children with allergic rhinitis |
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| المؤلفون: | Xiong‐Hui Wu, Si‐Jun Zhao, Wei‐Qing Huang, Li‐Hua Huang, Xin‐You Luo, Song‐Liang Long |
| المصدر: | Kaohsiung Journal of Medical Sciences, Vol 38, Iss 10, Pp 971-980 (2022) |
| بيانات النشر: | Wiley, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine (General) |
| مصطلحات موضوعية: | allergic rhinitis, GATA binding protein 3, long non‐coding RNA MALAT1, microRNA‐135b‐5p, T‐helper 2 differentiation, Medicine (General), R5-920 |
| الوصف: | Abstract Allergic rhinitis (AR) threatens patient survival. CD4+ T cells play key roles in AR progression. Long non‐coding RNAs (lncRNAs) are key regulators of cell differentiation. Therefore, we investigated the molecular mechanism of the lncRNA metastasis‐associated lung adenocarcinoma transcript 1 (MALAT1) in AR. Expression levels of MALAT1, microRNA (miR)‐135b‐5p, interleukin‐4 (IL‐4), and GATA‐binding protein 3 (GATA‐3) in the nasal mucosa of AR patients were quantified. CD4+ T cells were isolated from the peripheral blood of healthy volunteers and treated with ovalbumin (OVA) and Th2 inducers. After MALAT1 and miR‐135b‐5p levels changed in CD4+ T cells, the proportion of IL‐4‐expressing cells and the levels of IL‐4 and GATA‐3 in OVA‐induced CD4+ T cells were determined. Binding relationships among MALAT1, miR‐135b‐5p, and GATA‐3 were predicted and verified. Rescue experiments were performed to confirm the role of the MALAT1/miR‐135b‐5p/GATA‐3 axis in Th2 differentiation of CD4+ T cells. MALAT1, IL‐4, and GATA‐3 expression was upregulated, whereas miR‐135b‐5p expression was downregulated, in patients with AR. MALAT1 knockdown or miR‐135b‐5p overexpression in CD4+ T cells notably decreased the proportion of IL‐4‐expressing cells and downregulated GATA‐3 and IL‐4 expression in OVA‐induced CD4+ T cells. MALAT1 and GATA‐3 exhibited competitive binding toward miR‐135b‐5p. MALAT1 facilitated CD4+ T cell Th2 differentiation via the miR‐135b‐5p/GATA‐3 axis. MALAT1 facilitated AR development by facilitating CD4+ T cell Th2 differentiation via the miR‐135b‐5p/GATA‐3 axis. This study may provide guidance for clinical treatment of AR. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2410-8650 1607-551X |
| العلاقة: | https://doaj.org/toc/1607-551XTest; https://doaj.org/toc/2410-8650Test |
| DOI: | 10.1002/kjm2.12587 |
| الوصول الحر: | https://doaj.org/article/6ab69cce069c46858982c4a283a7770fTest |
| رقم الانضمام: | edsdoj.6ab69cce069c46858982c4a283a7770f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24108650 1607551X |
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| DOI: | 10.1002/kjm2.12587 |