دورية أكاديمية
Exosomal microRNA-15a from mesenchymal stem cells impedes hepatocellular carcinoma progression via downregulation of SALL4
| العنوان: | Exosomal microRNA-15a from mesenchymal stem cells impedes hepatocellular carcinoma progression via downregulation of SALL4 |
|---|---|
| المؤلفون: | Yu-Shui Ma, Ji-Bin Liu, Lan Lin, Hui Zhang, Jian-Jun Wu, Yi Shi, Cheng-You Jia, Dan-Dan Zhang, Fei Yu, Hui-Min Wang, Yu-Zhen Yin, Xiao-Hui Jiang, Pei-Yao Wang, Lin-Lin Tian, Ping-Sheng Cao, Xu-Ming Wu, Hai-Min Lu, Li-Peng Gu, Jia-Jia Zhang, Gu-Jun Cong, Pei Luo, Xiao-Ming Zhong, Bo Cai, Min-Xin Shi, Su-Qing Zhang, Liu Li, Wen-Jie Zhang, Yu Liu, Zhi-Zhen Li, Ting-Miao Wu, Zhi-Jun Wu, Gao-Ren Wang, Zhong-Wei Lv, Chang-Chun Ling, Kai-Jian Chu, Da Fu |
| المصدر: | Cell Death Discovery, Vol 7, Iss 1, Pp 1-11 (2021) |
| بيانات النشر: | Nature Publishing Group, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens LCC:Cytology |
| مصطلحات موضوعية: | Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282, Cytology, QH573-671 |
| الوصف: | Abstract Hepatocellular carcinoma (HCC) is a heterogeneous tumor with an increased incidence worldwide accompanied by high mortality and dismal prognosis. Emerging evidence indicates that mesenchymal stem cells (MSCs)-derived exosomes possess protective effects against various human diseases by transporting microRNAs (miRNAs or miRs). We aimed to explore the role of exosomal miR-15a derived from MSCs and its related mechanisms in HCC. Exosomes were isolated from transduced MSCs and co-incubated with Hep3B and Huh7 cells. miR-15a expression was examined by RT-qPCR in HCC cells, MSCs, and secreted exosomes. CCK-8, transwell, and flow cytometry were used to detect the effects of miR-15a or spalt-like transcription factor 4 (SALL4) on cell proliferative, migrating, invasive, and apoptotic properties. A dual-luciferase reporter gene assay was performed to validate the predicted targeting relationship of miR-15a with SALL4. Finally, in vivo experiments in nude mice were implemented to assess the impact of exosome-delivered miR-15a on HCC. The exosomes from MSCs restrained HCC cell proliferative, migrating, and invasive potentials, and accelerated their apoptosis. miR-15a was expressed at low levels in HCC cells and could bind to SALL4, thus curtailing the proliferative, migrating, and invasive abilities of HCC cells. Exosomes successfully delivered miR-15a to HCC cells. Exosomal miR-15a depressed tumorigenicity and metastasis of HCC tumors in vivo. Overall, exosomal miR-15a from MSCs can downregulate SALL4 expression and thereby retard HCC development. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2058-7716 |
| العلاقة: | https://doaj.org/toc/2058-7716Test |
| DOI: | 10.1038/s41420-021-00611-z |
| الوصول الحر: | https://doaj.org/article/6981ee02b88e4d75bd4b126f2e693ff0Test |
| رقم الانضمام: | edsdoj.6981ee02b88e4d75bd4b126f2e693ff0 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20587716 |
|---|---|
| DOI: | 10.1038/s41420-021-00611-z |