دورية أكاديمية
A multiplex method for detection of SARS-CoV-2 variants based on MALDI-TOF mass spectrometry
| العنوان: | A multiplex method for detection of SARS-CoV-2 variants based on MALDI-TOF mass spectrometry |
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| المؤلفون: | Ziyuan Zhao, Liying Sun, Liqin Wang, Xiaodong Li, Junping Peng |
| المصدر: | Biosafety and Health, Vol 5, Iss 2, Pp 101-107 (2023) |
| بيانات النشر: | Elsevier, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Infectious and parasitic diseases LCC:Public aspects of medicine |
| مصطلحات موضوعية: | COVID-19, SARS-CoV-2, Multiplexing capability, MALDI-TOF mass spectrometry, Viral variation, Infectious and parasitic diseases, RC109-216, Public aspects of medicine, RA1-1270 |
| الوصف: | The recent outbreak of the coronavirus disease 2019 (COVID-19) pandemic and the continuous evolution of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have highlighted the significance of new detection methods for global monitoring and prevention. Although quantitative reverse transcription PCR (RT-qPCR), the current gold standard for diagnosis, performs excellently in genetic testing, its multiplexing capability is limited because of the signal crosstalk of various fluorophores. Herein, we present a highly efficient platform which combines 17-plex assays with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), enabling the targeting of 14 different mutation sites of the spike gene. Diagnosis using a set of 324 nasopharyngeal swabs or sputum clinical samples with SARS-CoV-2 MS method was identical to that with the RT-qPCR. The detection consistency of mutation sites was 97.9% (47/48) compared to Sanger sequencing without cross-reaction with other respiratory-related pathogens. Therefore, the MS method is highly potent to track and assess SARS-CoV-2 changes in a timely manner, thereby aiding the continuous response to viral variation and prevention of further transmission. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2590-0536 |
| العلاقة: | http://www.sciencedirect.com/science/article/pii/S2590053623000216Test; https://doaj.org/toc/2590-0536Test |
| DOI: | 10.1016/j.bsheal.2023.02.003 |
| الوصول الحر: | https://doaj.org/article/dc68af5a72e54e3bbd008ad262068929Test |
| رقم الانضمام: | edsdoj.68af5a72e54e3bbd008ad262068929 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 25900536 |
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| DOI: | 10.1016/j.bsheal.2023.02.003 |