دورية أكاديمية
The mRNA expression levels of calpains and their activity in malignant and dysplastic epithelium of the upper respiratory tract
| العنوان: | The mRNA expression levels of calpains and their activity in malignant and dysplastic epithelium of the upper respiratory tract |
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| المؤلفون: | E. A. Sidenko, G. V. Kakurina, O. V. Cheremisina, L. V. Spirina, E. E. Shashova, D. A. Korshunov, I. V. Kondakova |
| المصدر: | Бюллетень сибирской медицины, Vol 20, Iss 2, Pp 88-94 (2021) |
| بيانات النشر: | Siberian State Medical University (Tomsk), 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | плоскоклеточный рак головы и шеи, дисплазия эпителия, активность кальпаинов, экспрессия мрнк capn1 и capn2, Medicine |
| الوصف: | Background. The calpain proteolytic system plays an important role in the development of cancer. Detection of early cancer in the upper respiratory tract is often challenging, as symptoms are largely non-specific, and most cases are diagnosed at an advanced stage.Aim. To identify candidate markers of transition from premalignant lesions to invasive carcinoma, we studied mRNA expression levels of CAPN1 and CAPN2 and the total activity of calpains in the tumor tissues of patients with head and neck squamous cell carcinoma (HNSCC) and in the epithelial dysplasia-affected tissues of patients with chronic diseases of the upper respiratory tract.Materials and methods. The study included 32 patients with HNSCC (Т1-3N0-1М0) and 12 patients with chronic diseases of the upper respiratory system associated with epithelial dysplasia. The expression levels of CAPN1 and CAPN2 were assessed using real-time polymerase chain reaction (PCR). The calpain activity was determined by hydrolysis of the fluorogenic Suc-LLVY-AMC oligopeptide.Results. The mRNA expression levels of CAPN1 and CAPN2 were respectively 3 and 4 times higher in the tumor tissue of patients with HNSCC than in the tissue of patients with endothelial dysplasia in the upper respiratory tract. The level of calpain activity was 4.4 times higher in patients with HNSCC than in patients with epithelial dysplasia of different severity.Conclusion. The elevated mRNA expression levels of CAPN1 and CAPN2 and their activity in the tumor tissues of patients with HNSCC compared to patients with chronic respiratory diseases associated with epithelial dysplasia are likely to characterize a high potential for transition from precancerous lesion to cancer. To clarify the role of calpains in the carcinogenesis of HNSCC, further studies of intact tissues using animal models are required. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Russian |
| تدمد: | 1682-0363 1819-3684 |
| العلاقة: | https://bulletin.ssmu.ru/jour/article/view/4383Test; https://doaj.org/toc/1682-0363Test; https://doaj.org/toc/1819-3684Test |
| DOI: | 10.20538/1682-0363-2021-2-88-94 |
| الوصول الحر: | https://doaj.org/article/66bdbf93deb74cd8b7faeec0e8b455e6Test |
| رقم الانضمام: | edsdoj.66bdbf93deb74cd8b7faeec0e8b455e6 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 16820363 18193684 |
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| DOI: | 10.20538/1682-0363-2021-2-88-94 |