دورية أكاديمية
Effectiveness of conjunctival bleb scarring by knock-down of heat shock protein 47 in rat model
| العنوان: | Effectiveness of conjunctival bleb scarring by knock-down of heat shock protein 47 in rat model |
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| المؤلفون: | Wei-Wei Wang, Hai-Yan Li, Huan-Huan Yan |
| المصدر: | International Journal of Ophthalmology, Vol 16, Iss 10, Pp 1589-1594 (2023) |
| بيانات النشر: | Press of International Journal of Ophthalmology (IJO PRESS), 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Ophthalmology |
| مصطلحات موضوعية: | heat shock protein 47, filtration surgery, conjunctival bleb, scar, transforming growth factor-β1, Ophthalmology, RE1-994 |
| الوصف: | AIM: To evaluate the effectiveness of knock-down of heat shock protein 47 (HSP47) on conjunctival bleb scarring in a rat model and its possible mechanism. METHODS: Male Sprague–Dawley rats were used for glaucoma filtration surgery (GFS) and were treated with either phosphate buffered solution, shControl, mitomycin C, or sh-HSP47 using a microsyringe immediately after GFS. The morphology of filtering blebs was observed postoperatively. The levels of HSP47 were analyzed at 2, 5, 8, and 11d after GFS via real time quantitative polymerase chain reaction (PCR) and Western blot. The silencing effect of HSP47, the expression of collagen I and III, and the potential signaling pathways of HSP47 during scarification were explored 11d post GFS. The protein levels of transforming growth factor-β1 (TGF-β1), phospho-Smad2 (pSmad2), phospho-Smad3 (p-Smad3), and phospho-p38 (p-p38) were also analyzed using Western blot. RESULTS: Sh-HSP47 treatment significantly prolonged the functional filtration bleb retention. The levels of HSP47 were increased significantly at 5, 8, and 11d postoperatively compared to the control group (P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2222-3959 2227-4898 |
| العلاقة: | http://ies.ijo.cn/en_publish/2023/10/20231005.pdfTest; https://doaj.org/toc/2222-3959Test; https://doaj.org/toc/2227-4898Test |
| DOI: | 10.18240/ijo.2023.10.05 |
| الوصول الحر: | https://doaj.org/article/e635047951224a3c9326d77ea7efd03aTest |
| رقم الانضمام: | edsdoj.635047951224a3c9326d77ea7efd03a |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22223959 22274898 |
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| DOI: | 10.18240/ijo.2023.10.05 |