دورية أكاديمية
Intraocular Microbiome in Diabetes and Diabetic Retinopathy: A Pilot Study
| العنوان: | Intraocular Microbiome in Diabetes and Diabetic Retinopathy: A Pilot Study |
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| المؤلفون: | Taraprasad Das, Shalem Raj Padakandla, Sisinthy Shivaji, Rajagopalaboopathi Jayasudha, Brijesh Takkar |
| المصدر: | Ophthalmology and Therapy, Vol 12, Iss 2, Pp 1109-1126 (2023) |
| بيانات النشر: | Adis, Springer Healthcare, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Ophthalmology |
| مصطلحات موضوعية: | Microbiome, Diabetes mellitus, Diabetic retinopathy, Gut retina axis, Prevention of blindness, Ophthalmology, RE1-994 |
| الوصف: | Abstract Introduction The objective of this study was to compare the microbiome in the aqueous humour and gut of people with diabetes mellitus (DM) with and without diabetic retinopathy (DR). Methods This was a prospective controlled study. The study included 17 people undergoing intraocular surgery in their naïve eyes. Stool samples were obtained in the perioperative period; aqueous humour samples of sufficient quantity were obtained in 12 people during intraocular surgery. Dietary information was obtained using a previously validated questionnaire. The gut and aqueous humour samples were assessed for microbiome using 16S rRNA gene sequencing coupled with QIIME and R software. Results Aqueous humour was analysed in 12 people: 4 each healthy controls, people with DM, and people with DR. There were minor differences at the phyla levels, but the aqueous humour microbiomes of healthy controls, DM, and DR formed three distinct clusters on heat map analysis with discriminatory genera. This genera-level clustering was more apparent for the intraocular than the gut microbiome. In people with DM and DR, we identified genera unique to the eye or the gut. There was a consistent reduction in the abundance of anti-inflammatory bacteria in people with DR than DM. Conclusions There is a difference in intraocular and gut microbiome regardless of disease or health. Our preliminary findings indicate distinctive features of the intraocular microbiome in people with DR compared with those without it. While this distinctiveness appears more evident in aqueous humour than in the gut, it needs further confirmation with larger studies. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2193-8245 2193-6528 |
| العلاقة: | https://doaj.org/toc/2193-8245Test; https://doaj.org/toc/2193-6528Test |
| DOI: | 10.1007/s40123-023-00660-w |
| الوصول الحر: | https://doaj.org/article/a5e58fbcd7134713a53b69f82d749f49Test |
| رقم الانضمام: | edsdoj.5e58fbcd7134713a53b69f82d749f49 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 21938245 21936528 |
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| DOI: | 10.1007/s40123-023-00660-w |