دورية أكاديمية
Simultaneous determination of emtricitabine and tenofovir disoproxil fumarate in pharmaceutical preparations using spectrophotometric, chemometric and chromatographic methods
| العنوان: | Simultaneous determination of emtricitabine and tenofovir disoproxil fumarate in pharmaceutical preparations using spectrophotometric, chemometric and chromatographic methods |
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| المؤلفون: | Dinçel Aysun, Gök-Topak Elif Damla, Onur Feyyaz |
| المصدر: | Journal of the Serbian Chemical Society, Vol 89, Iss 4, Pp 551-563 (2024) |
| بيانات النشر: | Serbian Chemical Society, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Chemistry |
| مصطلحات موضوعية: | tenofovir disoproxil fumarate, emtricitabine, first derivative, ratio, pls, hplc, Chemistry, QD1-999 |
| الوصف: | Simple, accurate and sensitive spectrophotometric, chemometric and chromatographic methods were used for the simultaneous determination of emtricitabine (ETC) and tenofovir disoproxil fumarate (TDF) in tablets. In 1st derivative spectrophotometry, the first derivative spectra of the solution of ETC and TDF in water were recorded as Δλ = 4 nm and the first derivative absorbances were measured at the zero-crossing points at 297.3 and 281.2 nm for ETC and TDF, respectively. In ratio of the 1st derivative spectrophotometry measurements were recorded at 239.0 and 270.2 nm for ETC and TDF, respectively. Then analytical signals were measured at the wavelengths corresponding to either maximum or minimum for both drugs. For these spectrophotometric methods Beer’s law is obeyed in the concentration range of 2–15 μg mL-1 for both drugs. As chemometric method, the PLS technique was used. In chromatographic method, the separation was achieved on a C18 column with DAD (262 nm) and isocratic elution of methanol, acetonitrile and 0.1 % orthophosphoric acid in the volume ratio of 40:40:20, respectively, containing the mobile phase. The mean recovery and the relative standard deviation of the methods were found as 97.51–100.17 % and 0.55–1.26 % respectively. All these methods were statistically compared, and they were successfully applied to a pharmaceutical preparation. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0352-5139 1820-7421 |
| العلاقة: | https://doaj.org/toc/0352-5139Test; https://doaj.org/toc/1820-7421Test |
| DOI: | 10.2298/JSC230909031D |
| الوصول الحر: | https://doaj.org/article/59d0f82c660d4a9f83b7731bf0df37c7Test |
| رقم الانضمام: | edsdoj.59d0f82c660d4a9f83b7731bf0df37c7 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 03525139 18207421 |
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| DOI: | 10.2298/JSC230909031D |