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CRISPR/Cas9 mediated CXCL4 knockout in human iPS cells of polycythemia vera patient with JAK2 V617F mutation

التفاصيل البيبلوغرافية
العنوان: CRISPR/Cas9 mediated CXCL4 knockout in human iPS cells of polycythemia vera patient with JAK2 V617F mutation
المؤلفون: Janik Boehnke, Salim Atakhanov, Marcelo A.S. Toledo, Herdit M. Schüler, Stephanie Sontag, Nicolas Chatain, Steffen Koschmieder, Tim H. Brümmendorf, Rafael Kramann, Martin Zenke
المصدر: Stem Cell Research, Vol 55, Iss , Pp 102490- (2021)
بيانات النشر: Elsevier, 2021.
سنة النشر: 2021
المجموعة: LCC:Biology (General)
مصطلحات موضوعية: Induced pluripotent stem cell, iPS cells, Hematopoiesis, CXCL4, PF4, JAK2 V617F, Biology (General), QH301-705.5
الوصف: The chemokine CXCL4/platelet factor 4 (PF4) gene, a key player in myelofibrosis, was knocked out by CRISPR/Cas9 in induced pluripotent stem cells (iPS cells) of a polycythemia vera (PV) patient with JAK2 V617F mutation. Two CXCL4KO iPS cell lines with and without JAK2 V617F mutation (UKAi002-B-1 and UKAi002-A-1, respectively) were generated. CXCL4KO iPS cells showed deletion of exon 1 and complete loss of CXCL4 protein. Pluripotency of iPS cells was confirmed by expression of pluripotency markers and trilineage differentiation. CXCL4KO iPS cells are expected to provide a valuable tool for investigating the role of CXCL4 in human diseases.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1873-5061
العلاقة: http://www.sciencedirect.com/science/article/pii/S1873506121003378Test; https://doaj.org/toc/1873-5061Test
DOI: 10.1016/j.scr.2021.102490
الوصول الحر: https://doaj.org/article/d5201e98c76548fb80312315e5bbd47dTest
رقم الانضمام: edsdoj.5201e98c76548fb80312315e5bbd47d
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:18735061
DOI:10.1016/j.scr.2021.102490