دورية أكاديمية
Evaluation of the Antiseizure Activity of Endemic Plant Halfordia kendack Guillaumin and Its Main Constituent, Halfordin, on a Zebrafish Pentylenetetrazole (PTZ)-Induced Seizure Model
| العنوان: | Evaluation of the Antiseizure Activity of Endemic Plant Halfordia kendack Guillaumin and Its Main Constituent, Halfordin, on a Zebrafish Pentylenetetrazole (PTZ)-Induced Seizure Model |
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| المؤلفون: | Adrianna Skiba, Ewelina Kozioł, Simon Vlad Luca, Barbara Budzyńska, Piotr Podlasz, Wietske Van Der Ent, Elham Shojaeinia, Camila V. Esguerra, Mohammed Nour, Laurence Marcourt, Jean-Luc Wolfender, Krystyna Skalicka-Woźniak |
| المصدر: | International Journal of Molecular Sciences, Vol 24, Iss 3, p 2598 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | liquid-liquid chromatography, coumarins, zebrafish, epilepsy, LFP, electrophysiology, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Epilepsy is a neurological disease that burdens over 50 million people worldwide. Despite the considerable number of available antiseizure medications, it is estimated that around 30% of patients still do not respond to available treatment. Herbal medicines represent a promising source of new antiseizure drugs. This study aimed to identify new drug lead candidates with antiseizure activity from endemic plants of New Caledonia. The crude methanolic leaf extract of Halfordia kendack Guillaumin (Rutaceae) significantly decreased (75 μg/mL and 100 μg/mL) seizure-like behaviour compared to sodium valproate in a zebrafish pentylenetetrazole (PTZ)-induced acute seizure model. The main coumarin compound, halfordin, was subsequently isolated by liquid-liquid chromatography and subjected to locomotor, local field potential (LFP), and gene expression assays. Halfordin (20 μM) significantly decreased convulsive-like behaviour in the locomotor and LFP analysis (by 41.4% and 60%, respectively) and significantly modulated galn, and penka gene expression. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| العلاقة: | https://www.mdpi.com/1422-0067/24/3/2598Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test |
| DOI: | 10.3390/ijms24032598 |
| الوصول الحر: | https://doaj.org/article/ed4c8b12f8d24430bb33a3ea713f6badTest |
| رقم الانضمام: | edsdoj.4c8b12f8d24430bb33a3ea713f6bad |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms24032598 |