دورية أكاديمية
Identification of two glycerophosphodiester phosphodiesterase genes in maize leaf phosphorus remobilization
| العنوان: | Identification of two glycerophosphodiester phosphodiesterase genes in maize leaf phosphorus remobilization |
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| المؤلفون: | Jingxin Wang, Wenbo Pan, Alexiy Nikiforov, William King, Wanting Hong, Weiwei Li, Yang Han, Jana Patton-Vogt, Jianbo Shen, Lingyun Cheng |
| المصدر: | Crop Journal, Vol 9, Iss 1, Pp 95-108 (2021) |
| بيانات النشر: | KeAi Communications Co., Ltd., 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Agriculture LCC:Agriculture (General) |
| مصطلحات موضوعية: | Glycerophosphodiester phosphodiesterases, Maize, Phosphate deficiency, Phosphorus remobilization, Phospholipid, Agriculture, Agriculture (General), S1-972 |
| الوصف: | Phosphate deficiency is one of the leading causes of crop productivity loss. Phospholipid degradation liberates phosphate to cope with phosphate deficiency. Glycerophosphodiester phosphodiesterases (GPX-PDEs) hydrolyse the intermediate products of phospholipid catabolism glycerophosphodiesters into glycerol-3-phosphate, a precursor of phosphate. However, the function of GPX-PDEs in phosphate remobilization in maize remains unclear. In the present study, we characterized two phosphate deficiency-inducible GPX-PDE genes, ZmGPX-PDE1 and ZmGPX-PDE5, in maize leaves. ZmGPX-PDE1 and ZmGPX-PDE5 were transcriptionally regulated by ZmPHR1, a well-described phosphate starvation-responsive transcription factor of the MYB family. Complementation of the yeast GPX-PDE mutant gde1∆ indicated that ZmGPX-PDE1 and ZmGPX-PDE5 functioned as GPX-PDEs, suggesting their roles in phosphate recycling from glycerophosphodiesters. In vitro enzyme assays showed that ZmGPX-PDE1 and ZmGPX-PDE5 catalysed glycerophosphodiester degradation with different substrate preferences for glycerophosphoinositol and glycerophosphocholine, respectively. ZmGPX-PDE1 was upregulated during leaf senescence, and more remarkably, loss of ZmGPX-PDE1 in maize compromised the remobilization of phosphorus from senescing leaves to young leaves, resulting in a stay-green phenotype under phosphate starvation. These results suggest that ZmGPX-PDE1 catalyses the degradation of glycerophosphodiesters in maize, promoting phosphate recycling from senescing leaves to new leaves. This mechanism is crucial for improving phosphorus utilization efficiency in crops. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2214-5141 |
| العلاقة: | http://www.sciencedirect.com/science/article/pii/S2214514120300799Test; https://doaj.org/toc/2214-5141Test |
| DOI: | 10.1016/j.cj.2020.05.004 |
| الوصول الحر: | https://doaj.org/article/498f2d8a66244c47aef169b47f241ec6Test |
| رقم الانضمام: | edsdoj.498f2d8a66244c47aef169b47f241ec6 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22145141 |
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| DOI: | 10.1016/j.cj.2020.05.004 |