دورية أكاديمية
Pro-inflammatory activity of long noncoding RNA FOXD2-AS1 in Achilles tendinopathy
| العنوان: | Pro-inflammatory activity of long noncoding RNA FOXD2-AS1 in Achilles tendinopathy |
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| المؤلفون: | Xiaoting Ke, Wenjie Zhang |
| المصدر: | Journal of Orthopaedic Surgery and Research, Vol 18, Iss 1, Pp 1-9 (2023) |
| بيانات النشر: | BMC, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Orthopedic surgery LCC:Diseases of the musculoskeletal system |
| مصطلحات موضوعية: | Long noncoding RNA FOXD2-AS1, microRNA-21-3p, PTEN, PI3K/AKT signaling pathway, Achilles tendinopathy, Inflammatory response, Orthopedic surgery, RD701-811, Diseases of the musculoskeletal system, RC925-935 |
| الوصف: | Abstract Achilles tendinopathy is a prevalent clinical problem that plagues athletes and general populations. Achilles tendon healing is a complex process, and so far, there is no successful long-term solution to Achilles tendinopathy in the field of microsurgery due to its poor natural regeneration ability. Limitations in understanding the pathogenesis of Achilles tendon development and Achilles tendon injury hinder clinical treatment developments. There is an increasing demand for innovative conservative treatments that can improve Achilles tendon injury. In this study, a Sprague–Dawley rat model of Achilles tendinopathy was established. Lentiviral vectors that interfere with the expression of FOXD2-AS1, miR-21-3p, or PTEN were injected every 3 days. Rats were euthanized after 3 weeks, and the effect of FOXD2-AS1, miR-21-3p, or PTEN on Achilles tendon healing was analyzed by histological observation, biomechanical test, and examinations of inflammatory factors and tendon markers. As measured, downregulating FOXD2-AS1 or upregulating miR-21-3p improved histological structure, suppressed inflammation, promoted the expression of tendon markers, and optimized the biomechanical properties of Achilles tendon. Upregulating PTEN was capable of reversing the promoting effect of inhibition of FOXD2-AS1 on Achilles tendon healing. As concluded, deficiency of FOXD2-AS1 accelerates the healing of Achilles tendon injury and improves tendon degeneration by regulating the miR-21-3p/PTEN axis and promoting the activation of the PI3K/AKT signaling pathway. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1749-799X |
| العلاقة: | https://doaj.org/toc/1749-799XTest |
| DOI: | 10.1186/s13018-023-03681-0 |
| الوصول الحر: | https://doaj.org/article/47fd0e33d76b4ea6a32665cc6474ab26Test |
| رقم الانضمام: | edsdoj.47fd0e33d76b4ea6a32665cc6474ab26 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1749799X |
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| DOI: | 10.1186/s13018-023-03681-0 |