دورية أكاديمية
Molecular characterization, expression pattern and immunologic function of CD82a in large yellow croaker (Larimichthys crocea)
| العنوان: | Molecular characterization, expression pattern and immunologic function of CD82a in large yellow croaker (Larimichthys crocea) |
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| المؤلفون: | Yue Liu, Yulin Bai, Sijing Chen, Fei Pu, Yaxian Li, Hongshu Chi, Zaiyu Zheng, Peng Xu, Tao Zhou |
| المصدر: | Frontiers in Immunology, Vol 15 (2024) |
| بيانات النشر: | Frontiers Media S.A., 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Immunologic diseases. Allergy |
| مصطلحات موضوعية: | Larimichthys crocea, Pseudomonas plecoglossicida, CD82a, apoptosis, antigen presentation, Immunologic diseases. Allergy, RC581-607 |
| الوصف: | Visceral white spot disease (VWND) caused by Pseudomonas plecoglossicida poses a major threat to the sustainable development of large yellow croaker (Larimichthys crocea) aquaculture. Genome-wide association analysis (GWAS) and RNA-seq research indicated that LcCD82a play an important role in resistance to visceral white spot disease in L. crocea, but the molecular mechanism of LcCD82a response to P. plecoglossicida infection is still unclear. In this study, we cloned and validated the Open Reading Frame (ORF) sequence of LcCD82a and explored the expression profile of LcCD82a in various tissues of L.crocea. In addition, two different transcript variants (LcCD82a-L and LcCD82a-S) of LcCD82a were identified that exhibit alternative splicing patterns after P. plecoglossicida infection, which may be closely related to the immune regulation during pathogenetic process of VWND. In order to explore the function of LcCD82a, we purified the recombinant protein of LcCD82a-L and LcCD82a-S. The bacterial agglutination and apoptosis function analysis showed that LcCD82a may involve in extracellular bacterial recognition, agglutination, and at the same time participate in the process of antigen presentation and induction of cell apoptosis. Collectively, our studies demonstrate that LcCD82a plays a crucial role in regulating apoptosis and antimicrobial immunity. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1664-3224 87424622 |
| العلاقة: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1301877/fullTest; https://doaj.org/toc/1664-3224Test |
| DOI: | 10.3389/fimmu.2024.1301877 |
| الوصول الحر: | https://doaj.org/article/3e91cd96abc9483e874246228b42d439Test |
| رقم الانضمام: | edsdoj.3e91cd96abc9483e874246228b42d439 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16643224 87424622 |
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| DOI: | 10.3389/fimmu.2024.1301877 |