دورية أكاديمية

Short-term treatment with Uncaria tomentosa aggravates the injury phenotype in mdx mice

التفاصيل البيبلوغرافية
العنوان: Short-term treatment with Uncaria tomentosa aggravates the injury phenotype in mdx mice
المؤلفون: David Feder, Túlio de Almeida Hermes, Lucas Prezotto Giordani, Bruno Machado Bertassoli, Giuliana Petri, Fabio Perazzo, Fernando Luiz Affonso Fonseca, Alzira Alves de Siqueira Carvalho
المصدر: ABCS Health Sciences (2024)
بيانات النشر: Faculdade de Medicina do ABC, 2024.
سنة النشر: 2024
المجموعة: LCC:Medicine
مصطلحات موضوعية: Uncaria tomentosa, Duchenne muscular dystrophy, mdx mice, neuromuscular, myotoxic, Medicine
الوصف: Introduction: Uncaria tomentosa (Willd. ex Roem. & Schult.) DC. (Rubiaceae) or UT is a medicinal plant with antiviral, antimutagenic, anti-inflammatory and antioxidant properties. Duchenne muscular dystrophy (DMD) is a severe muscle wasting disease caused by mutations in the dystrophin gene; this deficiency leads to sarcolemma instability, inflammation, muscle degeneration and fibrosis. Objective: Considering the importance of inflammation to dystrophy progression and the anti-inflammatory activity of UT, in the present study we evaluated whether oral administration of UT extract would ameliorate dystrophy in the mdx mice, a DMD model. Methods: Eight-week-old male mdx mice were submitted to 200 mg/kg body weight daily UT oral administration for 6 weeks. General histopathology was analysed, and muscle tumor necrosis factor α, transforming growth factor-β, myostatin and osteopontin transcript levels were assessed. The ability of mice to sustain limb tension to oppose their gravitational force was measured. Data were analysed with the unpaired Student’s t-test. Results: Morphologically, both untreated and UT-treated animals exhibited internalised nuclei, increased endomysial connective tissue and variations in muscle fibre diameters. Body weight and muscle strength were significantly reduced in the UT-treated animals. Blood creatine kinase was higher in UT-treated compared to untreated animals. In tibialis anterior, myostatin, transcript was more highly expressed in the UT-treated while in the diaphragm muscle, transforming growth factor-β transcripts were less expressed in the UT-treated. Conclusion: While previous studies identified anti-inflammatory, antiproliferative and anticarcinogenic UT effects, the extract indicates worsening of dystrophic muscles phenotype after short-term treatment in mdx mice.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
Portuguese
تدمد: 2318-4965
2357-8114
العلاقة: https://www.portalnepas.org.br/abcshs/article/view/2058Test; https://doaj.org/toc/2318-4965Test; https://doaj.org/toc/2357-8114Test
DOI: 10.7322/abcshs.2022018.2058
الوصول الحر: https://doaj.org/article/39f956ad9cb6487782c1f4bcd7bf9036Test
رقم الانضمام: edsdoj.39f956ad9cb6487782c1f4bcd7bf9036
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:23184965
23578114
DOI:10.7322/abcshs.2022018.2058