دورية أكاديمية
Interaction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitro
| العنوان: | Interaction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitro |
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| المؤلفون: | Erick Vaz Guimarães, Laís de Carvalho, Helene Santos Barbosa |
| المصدر: | Memorias do Instituto Oswaldo Cruz, Vol 104, Iss 2, Pp 170-174 (2009) |
| بيانات النشر: | Fundação Oswaldo Cruz (FIOCRUZ), 2009. |
| سنة النشر: | 2009 |
| المجموعة: | LCC:Microbiology LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | Toxoplasma gondi, tissue cysts, bradyzoites, cystogenesis, skeletal muscle cells, Microbiology, QR1-502, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Infection by the protozoan parasite Toxoplasma gondii is widely prevalent in humans and animals. To prevent human infection, all meat should be well cooked before consumption, since the parasite is present in skeletal muscle. In this context, the use of skeletal muscle cells (SkMCs) as a cellular model opens up new approaches to investigate T. gondii-host cell interactions. Immunofluorescent detection of proteins that are stage-specific for bradyzoites indicated that complete cystogenesis of T. gondii in in vitro cultures of SkMCs occurs after 96 h of infection. Ultrastructural analysis showed that, after 48 h of interaction, there were alterations on the parasitophorous vacuole membrane, including greater thickness and increased electron density at the inner face of the membrane. The present study demonstrates the potential use of primary cultures of SkMCs to evaluate different molecular aspects of T. gondii invasion and cystogenesis and presents a promising in vitro model for the screening of drug activities toward tissue cysts and bradyzoites. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0074-0276 1678-8060 |
| العلاقة: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000200007Test; https://doaj.org/toc/0074-0276Test; https://doaj.org/toc/1678-8060Test |
| DOI: | 10.1590/S0074-02762009000200007 |
| الوصول الحر: | https://doaj.org/article/37003942fe52437a8e963094263eda88Test |
| رقم الانضمام: | edsdoj.37003942fe52437a8e963094263eda88 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 00740276 16788060 |
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| DOI: | 10.1590/S0074-02762009000200007 |