دورية أكاديمية
Intrahepatic transcriptomics reveals gene signatures in chronic hepatitis B patients responded to interferon therapy
| العنوان: | Intrahepatic transcriptomics reveals gene signatures in chronic hepatitis B patients responded to interferon therapy |
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| المؤلفون: | Ning Li, Kangkang Yu, Minhui Dong, Jinyu Wang, Feifei Yang, Haoxiang Zhu, Jie Yu, Jingshu Yang, Wentao Xie, Bidisha Mitra, Richeng Mao, Feizhen Wu, Haitao Guo, Jiming Zhang |
| المصدر: | Emerging Microbes and Infections, Vol 11, Iss 1, Pp 1876-1889 (2022) |
| بيانات النشر: | Taylor & Francis Group, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Infectious and parasitic diseases LCC:Microbiology |
| مصطلحات موضوعية: | Chronic hepatitis B, interferon therapy, liver biopsy, transcriptome, HBeAg, Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502 |
| الوصف: | Chronic hepatitis B virus (HBV) infection remains a substantial public health burden worldwide. Alpha-interferon (IFNα) is one of the two currently approved therapies for chronic hepatitis B (CHB), to explore the mechanisms underlying IFNα treatment response, we investigated baseline and 24-week on-treatment intrahepatic gene expression profiles in 21 CHB patients by mRNA-seq. The data analyses demonstrated that PegIFNα treatment significantly induced antiviral responses. Responders who achieved HBV DNA loss and HBeAg or HBsAg seroconversion displayed higher fold change and larger number of up-regulated interferon-stimulated genes (ISGs). Interestingly, lower expression levels of certain ISGs were observed in responders in their baseline biopsy samples. In HBeAg+ patients, non-responders had relative higher baseline HBeAg levels than responders. More importantly, HBeAg− patients showed higher HBsAg loss rate than HBeAg+ patients. Although a greater fold change of ISGs was observed in HBeAg− patients than HBeAg+ patients, upregulation of ISGs in HBeAg+ responders exceeded HBeAg− responders. Notably, PegIFNα treatment increased monocyte and mast cell infiltration, but decreased CD8 T cell and M1 macrophage infiltration in both responders and non-responders, while B cell infiltration was increased only in responders. Moreover, co-expression analysis identified ribosomal proteins as critical players in antiviral response. The data also indicate that IFNα may influence the production of viral antigens associated with endoplasmic reticulum. Collectively, the intrahepatic transcriptome analyses in this study enriched our understanding of IFN-mediated antiviral effects in CHB patients and provided novel insights into the development of potential strategies to improve IFNα therapy. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 22221751 2222-1751 74782886 |
| العلاقة: | https://doaj.org/toc/2222-1751Test |
| DOI: | 10.1080/22221751.2022.2100831 |
| الوصول الحر: | https://doaj.org/article/34ac08a2fed74782886862be48e5288dTest |
| رقم الانضمام: | edsdoj.34ac08a2fed74782886862be48e5288d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22221751 74782886 |
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| DOI: | 10.1080/22221751.2022.2100831 |