دورية أكاديمية
Congenital SARS-CoV-2 Infection in Two Neonates with Confirmation by Viral Culture of the Placenta in One Case
| العنوان: | Congenital SARS-CoV-2 Infection in Two Neonates with Confirmation by Viral Culture of the Placenta in One Case |
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| المؤلفون: | Joseph V. Vayalumkal, Amuchou S. Soraisham, Ayman Abou Mehrem, Anirban Ghosh, Jessica K. E. Dunn, Kevin Fonseca, Hong Zhou, Byron M. Berenger, Elaine S. Chan, Marie-Anne Brundler, Yi-Chan Lin, David H. Evans, Sharon Rousso, Verena Kuret, John M. Conly |
| المصدر: | Viruses, Vol 15, Iss 6, p 1310 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Microbiology |
| مصطلحات موضوعية: | congenital, transplacental, SARS-CoV-2, neonatal, pregnancy, viral culture, Microbiology, QR1-502 |
| الوصف: | Congenital infections with SARS-CoV-2 are uncommon. We describe two confirmed congenital SARS-CoV-2 infections using descriptive, epidemiologic and standard laboratory methods and in one case, viral culture. Clinical data were obtained from health records. Nasopharyngeal (NP) specimens, cord blood and placentas when available were tested by reverse transcriptase real-time PCR (RT-PCR). Electron microscopy and histopathological examination with immunostaining for SARS-CoV-2 was conducted on the placentas. For Case 1, placenta, umbilical cord, and cord blood were cultured for SARS-CoV-2 on Vero cells. This neonate was born at 30 weeks, 2 days gestation by vaginal delivery. RT-PCR tests were positive for SARS-CoV-2 from NP swabs and cord blood; NP swab from the mother and placental tissue were positive for SARS-CoV-2. Placental tissue yielded viral plaques with typical morphology for SARS-CoV-2 at 2.8 × 102 pfu/mL confirmed by anti-spike protein immunostaining. Placental examination revealed chronic histiocytic intervillositis with trophoblast necrosis and perivillous fibrin deposition in a subchorionic distribution. Case 2 was born at 36 weeks, 4 days gestation. RT-PCR tests from the mother and infant were all positive for SARS-CoV-2, but placental pathology was normal. Case 1 may be the first described congenital case with SARS-CoV-2 cultivated directly from placental tissue. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 15061310 1999-4915 |
| العلاقة: | https://www.mdpi.com/1999-4915/15/6/1310Test; https://doaj.org/toc/1999-4915Test |
| DOI: | 10.3390/v15061310 |
| الوصول الحر: | https://doaj.org/article/33b025c56bff4f83a2ff7b75132ac01fTest |
| رقم الانضمام: | edsdoj.33b025c56bff4f83a2ff7b75132ac01f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 15061310 19994915 |
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| DOI: | 10.3390/v15061310 |