دورية أكاديمية

Mucosal molecular pattern of tissue transglutaminase and interferon gamma in suspected seronegative celiac disease at marsh 1 and 0 stages

التفاصيل البيبلوغرافية
العنوان: Mucosal molecular pattern of tissue transglutaminase and interferon gamma in suspected seronegative celiac disease at marsh 1 and 0 stages
المؤلفون: Enzo Ierardi, Annacinzia Amoruso, Floriana Giorgio, Mariabeatrice Principi, Giuseppe Losurdo, Domenico Piscitelli, Francesca Buffelli, Maria G Fiore, Antonio Mongelli, Nicola M Castellaneta, Antonio Giangaspero, Vincenzo De Francesco, Lucia Montenegro, Alfredo Di Leo
المصدر: The Saudi Journal of Gastroenterology, Vol 21, Iss 6, Pp 379-385 (2015)
بيانات النشر: Wolters Kluwer Medknow Publications, 2015.
سنة النشر: 2015
المجموعة: LCC:Diseases of the digestive system. Gastroenterology
مصطلحات موضوعية: Celiac disease, interferon gamma, intraepithelial lymphocytes, seronegative celiac disease, tissue transglutaminase, Diseases of the digestive system. Gastroenterology, RC799-869
الوصف: Background/Aim: In celiac disease (CD), there is increased mRNA coding for tissue transglutaminase (tTG) and interferon gamma (IFNα). In seronegative celiac patients, the mucosal immune complexes anti-tTG IgA/tTG are found. We assayed tTG- and IFNα-mRNA in the mucosa of patients with a clinical suspicion of seronegative CD and correlated the values with intraepithelial CD3 lymphocytes (IELs). Materials and Methods: Distal duodenum specimens from 67 patients were retrieved and re-evaluated for immunohistochemically proven CD3 IELs. Five 10 μm sections were used for the extraction and assay of tTG and IFNα coding mRNA levels using reverse transcriptase real-time polymerase chain reaction (RT-PCR). Samples from 15 seropositive CD patients and 15 healthy subjects were used as positive and negative controls. Results were expressed as fold-change. Results: Our series was divided into three groups based on IEL count: >25 (14 patients: group A), 15–25 (26 patients: group B), and 0–15 (27 patients: Group C). tTG-mRNA levels were (mean ± SD): CD = 9.8 ± 2.6; group A = 10.04 ± 4.7; group B = 4.99 ± 2.3; group C = 2.26 ± 0.8, controls = 1.04 ± 0.2 (CD = group A > group B > group C = controls). IFNα-mRNA levels were: CD = 13.4 ± 3.6; group A = 7.28 ± 3.6; group B = 4.45 ± 2.9; group C = 2.06 ± 1.21, controls = 1.04 ± 0.4. Conclusions: Our results suggest that tTG- and IFNγmRNA levels are increased in both seropositive and potential seronegative patients with CD, showing a strong correlation with the CD3 IEL count at stage Marsh 1. An increase in both molecules is found even when IELs are in the range 15–25 (Marsh 0), suggesting the possibility of a "gray zone" inhabited by patients which should be closely followed up in gluten-related disorders.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1319-3767
1998-4049
العلاقة: http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2015;volume=21;issue=6;spage=379;epage=385;aulast=IerardiTest; https://doaj.org/toc/1319-3767Test; https://doaj.org/toc/1998-4049Test
DOI: 10.4103/1319-3767.167189
الوصول الحر: https://doaj.org/article/3378cfdb89e6412e9ef85c7589c123eaTest
رقم الانضمام: edsdoj.3378cfdb89e6412e9ef85c7589c123ea
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:13193767
19984049
DOI:10.4103/1319-3767.167189