دورية أكاديمية
Advance typing of Vibrio parahaemolyticus through the mtlA and aer gene: A high-resolution, cost-effective approach
| العنوان: | Advance typing of Vibrio parahaemolyticus through the mtlA and aer gene: A high-resolution, cost-effective approach |
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| المؤلفون: | Lei Zhou, Danlei Liu, Yongqiang Zhu, Zilong Zhang, Shiwen Chen, Guoping Zhao, Huajun Zheng |
| المصدر: | Heliyon, Vol 10, Iss 3, Pp e25642- (2024) |
| بيانات النشر: | Elsevier, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Science (General) LCC:Social sciences (General) |
| مصطلحات موضوعية: | Vibrio parahaemolyticus, Pan-genome, Core genome, Typing, mtlA, aer, Science (General), Q1-390, Social sciences (General), H1-99 |
| الوصف: | Vibrio parahaemolyticus is a significant cause of foodborne illness, and its incidence worldwide is on the rise. It is thus imperative to develop a straightforward and efficient method for typing strains of this pathogen. In this study, we conducted a pangenome analysis of 75 complete genomes of V. parahaemolyticus and identified the core gene mtlA with the highest degree of variation, which distinguished 44 strains and outperformed traditional seven-gene-based MLST when combined with aer, another core gene with high degree of variation. The mtlA gene had higher resolution to type strains with a close relationship compared to the traditional MLST genes in the phylogenetic tree built by core genomes. Strong positive selection was also detected in the gene mtlA (ω > 1), representing adaptive and evolution in response to the environment. Therefore, the panel of gene mtlA and aer may serve as a tool for the typing of V. parahaemolyticus, potentially contributing to the prevention and control of this foodborne disease. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2405-8440 77359968 |
| العلاقة: | http://www.sciencedirect.com/science/article/pii/S2405844024016736Test; https://doaj.org/toc/2405-8440Test |
| DOI: | 10.1016/j.heliyon.2024.e25642 |
| الوصول الحر: | https://doaj.org/article/3326530c30894d4780de77359968e865Test |
| رقم الانضمام: | edsdoj.3326530c30894d4780de77359968e865 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24058440 77359968 |
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| DOI: | 10.1016/j.heliyon.2024.e25642 |