دورية أكاديمية
Global expression of noncoding RNome reveals dysregulation of small RNAs in patients with HTLV-1–associated adult T-cell leukemia: a pilot study
| العنوان: | Global expression of noncoding RNome reveals dysregulation of small RNAs in patients with HTLV-1–associated adult T-cell leukemia: a pilot study |
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| المؤلفون: | Andrezza Nascimento, Daniela Raguer Valadão de Souza, Rodrigo Pessôa, Anna Julia Pietrobon, Youko Nukui, Juliana Pereira, Jorge Casseb, Augusto César Penalva de Oliveira, Paula Loureiro, Alberto José da Silva Duarte, Patricia Bianca Clissa, Sabri Saeed Sanabani |
| المصدر: | Infectious Agents and Cancer, Vol 16, Iss 1, Pp 1-13 (2021) |
| بيانات النشر: | BMC, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | Small RNA, HTLV-1, T cell antigen receptor, Asymptomatic carriers, Massively parallel sequencing, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Abstract Background Adult T cell lymphoma/leukemia (ATLL) is a peripheral T-cell neoplasm caused by human T-cell lymphotropic virus-1 (HTLV-1). Small RNAs (sRNAs), including microRNAs (miRNAs), play a pivotal role in the initiation and development of hematological malignancies and may represent potential therapeutic target molecules. However, little is known about how these molecules impact the pathogenesis of ATLL. In this study, we aimed to identify sRNA expression signatures associated with ATLL and to investigate their potential implication in the pathophysiology of the disease. Methods Small-RNAseq analysis was performed in peripheral blood mononuclear cells from HTLV-1- associated ATLL (n = 10) in comparison to asymptomatic carriers (n = 8) and healthy controls (n = 5). Sequencing was carried out using the Illumina MiSeq platform, and the deregulation of selected miRNAs was validated by real-time PCR. Pathway analyses of most deregulated miRNA were performed and their global profiling was combined with transcriptome data in ATLL. Results The sequencing identified specific sRNAs signatures associated with ATLL patients that target pathways relevant in ATLL, such as the transforming growth factor-(βTGF-β), Wnt, p53, apoptosis, and mitogen-activated protein kinase (MAPK) signaling cascades. Network analysis revealed several miRNAs regulating highly connected genes within the ATLL transcriptome. miR-451-3p was the most downregulated miRNA in active patients. Conclusions Our findings shed light on the expression of specific sRNAs in HTLV-1 associated ATLL, which may represent promising candidates as biomarkers that help monitor the disease activity. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1750-9378 |
| العلاقة: | https://doaj.org/toc/1750-9378Test |
| DOI: | 10.1186/s13027-020-00343-2 |
| الوصول الحر: | https://doaj.org/article/a2bd03b12e0d429ebaf0e729d9dfacebTest |
| رقم الانضمام: | edsdoj.2bd03b12e0d429ebaf0e729d9dfaceb |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17509378 |
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| DOI: | 10.1186/s13027-020-00343-2 |