دورية أكاديمية
Nuclear localization of histamine receptor 2 in primary human lymphatic endothelial cells
| العنوان: | Nuclear localization of histamine receptor 2 in primary human lymphatic endothelial cells |
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| المؤلفون: | Sarit Pal, Anatoliy Gashev, Debarshi Roy |
| المصدر: | Biology Open, Vol 11, Iss 7 (2022) |
| بيانات النشر: | The Company of Biologists, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | gpcr, histamine receptor 2, nuclear localization, Science, Biology (General), QH301-705.5 |
| الوصف: | Histamine exerts its physiological functions through its four receptor subtypes. In this work, we report the subcellular localization of histamine receptor 2 (H2R), a G protein-coupled receptor (GPCR), which is expressed in a wide variety of cell and tissue types. A growing number of GPCRs have been shown to be localized in the nucleus and contribute toward transcriptional regulation. In this study, for the first time, we demonstrate the nuclear localization of H2R in lymphatic endothelial cells. In the presence of its ligand, we show significant upregulation of H2R nuclear translocation kinetics. Using fluorescently tagged histamine, we explored H2R-histamine binding interaction, which exhibits a critical role in this translocation event. Altogether, our results highlight the previously unrecognized nuclear localization pattern of H2R. At the same time, H2R as a GPCR imparts many unresolved questions, such as the functional relevance of this localization, and whether H2R can contribute directly to transcriptional regulation and can affect lymphatic specific gene expression. H2R blockers are commonly used medications that recently have shown significant side effects. Therefore, it is imperative to understand the precise molecular mechanism of H2R biology. In this aspect, our present data shed new light on the unexplored H2R signaling mechanisms. This article has an associated First Person interview with the first author of the paper. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2046-6390 |
| العلاقة: | http://bio.biologists.org/content/11/7/bio059191Test; https://doaj.org/toc/2046-6390Test |
| DOI: | 10.1242/bio.059191 |
| الوصول الحر: | https://doaj.org/article/d283a500b73b4da5b799430956c6f8dcTest |
| رقم الانضمام: | edsdoj.283a500b73b4da5b799430956c6f8dc |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20466390 |
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| DOI: | 10.1242/bio.059191 |