دورية أكاديمية
CSChighE-cadherinlow immunohistochemistry panel predicts poor prognosis in oral squamous cell carcinoma
| العنوان: | CSChighE-cadherinlow immunohistochemistry panel predicts poor prognosis in oral squamous cell carcinoma |
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| المؤلفون: | Rafael Carneiro Ortiz, Nádia Ghinelli Amôr, Luciana Mieli Saito, Mariana Rodrigues Santesso, Nathália Martins Lopes, Rodrigo Fonseca Buzo, Angélica Cristina Fonseca, Gleyson Kleber Amaral-Silva, Raquel Ajub Moyses, Camila Oliveira Rodini |
| المصدر: | Scientific Reports, Vol 14, Iss 1, Pp 1-13 (2024) |
| بيانات النشر: | Nature Portfolio, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Cancer stem cells, Epithelial-mesenchymal transition, Digital pathology, Immunohistochemistry, Oral cancer, Metastasis, Medicine, Science |
| الوصف: | Abstract Identifying marker combinations for robust prognostic validation in primary tumour compartments remains challenging. We aimed to assess the prognostic significance of CSC markers (ALDH1, CD44, p75NTR, BMI-1) and E-cadherin biomarkers in OSCC. We analysed 94 primary OSCC and 67 metastatic lymph node samples, including central and invasive tumour fronts (ITF), along with clinicopathological data. We observed an increase in ALDH1+/CD44+/BMI-1- tumour cells in metastatic lesions compared to primary tumours. Multivariate analysis highlighted that elevated p75NTR levels (at ITF) and reduced E-cadherin expression (at the tumour centre) independently predicted metastasis, whilst ALDH1high exhibited independent predictive lower survival at the ITF, surpassing the efficacy of traditional tumour staging. Then, specifically at the ITF, profiles characterized by CSChighE-cadherinlow (ALDH1highp75NTRhighE-cadherinlow) and CSCintermediateE-cadherinlow (ALDH1 or p75NTRhighE-cadherinlow) were significantly associated with worsened overall survival and increased likelihood of metastasis in OSCC patients. In summary, our study revealed diverse tumour cell profiles in OSCC tissues, with varying CSC and E-cadherin marker patterns across primary tumours and metastatic sites. Given the pivotal role of reduced survival rates as an indicator of unfavourable prognosis, the immunohistochemistry profile identified as CSChighE-cadherinlow at the ITF of primary tumours, emerges as a preferred prognostic marker closely linked to adverse outcomes in OSCC. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2045-2322 |
| العلاقة: | https://doaj.org/toc/2045-2322Test |
| DOI: | 10.1038/s41598-024-55594-5 |
| الوصول الحر: | https://doaj.org/article/d277233b7c0a4f9ea0b8b174b1351955Test |
| رقم الانضمام: | edsdoj.277233b7c0a4f9ea0b8b174b1351955 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-024-55594-5 |