دورية أكاديمية
Nesfatin-1 attenuated lipopolysaccharide-induced inflammatory response and senescence in human dental pulp cells
| العنوان: | Nesfatin-1 attenuated lipopolysaccharide-induced inflammatory response and senescence in human dental pulp cells |
|---|---|
| المؤلفون: | Lili Zhang, Bo Pang, Rong Wang, Bin Yang, Xubei Jia |
| المصدر: | Heliyon, Vol 10, Iss 12, Pp e32108- (2024) |
| بيانات النشر: | Elsevier, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Science (General) LCC:Social sciences (General) |
| مصطلحات موضوعية: | Gingivitis, LPS, SIRT1, SA-β-gal, Human dental pulp cells, Science (General), Q1-390, Social sciences (General), H1-99 |
| الوصف: | Lipopolysaccharide (LPS)-triggered damage in human dental pulp cells (hDPCs) is associated with the progression of gingivitis, which is inflammation of the gingival tissue. Nesfatin-1 is a peptide secreted by neurons and peripheral tissues. Here, we report a novel property of Nesfatin-1 in ameliorating LPS-induced inflammatory response and senescence in hDPCs. First, we demonstrate that Nesfatin-1 repressed LPS-triggered expression of inflammatory factors. Secondly, Nesfatin-1 restored telomerase activity and the expression of human telomerase reverse transcriptase (hTERT) and telomeric repeat binding factor 2 (TERF2) against LPS. Senescence-associated β-galactosidase (SA-β-gal) staining assay revealed that Nesfatin-1 attenuated LPS-induced cellular senescence in hDPCs. We also found that Nesfatin-1 increased telomerase activity in LPS-challenged hDPCs. It is also shown that Nesfatin-1 reduced the expression of plasminogen activator inhibitor-1 (PAI-1) and p16. Additionally, LPS stimulation reduced the expression of SIRT1, which was rescued by Nesfatin-1. However, the silencing of sirtuin1 (SIRT1) abrogated the protective property of Nesfatin-1 in preventing cellular senescence, implying that the function of Nesfatin-1 is regulated by SIRT1. Taken together, our findings suggest that Nesfatin-1 might possess a protective effect against gingivitis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2405-8440 |
| العلاقة: | http://www.sciencedirect.com/science/article/pii/S2405844024081398Test; https://doaj.org/toc/2405-8440Test |
| DOI: | 10.1016/j.heliyon.2024.e32108 |
| الوصول الحر: | https://doaj.org/article/273aa445de2a48d59c5cbda046a9d0d1Test |
| رقم الانضمام: | edsdoj.273aa445de2a48d59c5cbda046a9d0d1 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24058440 |
|---|---|
| DOI: | 10.1016/j.heliyon.2024.e32108 |