The cells and molecules involved in the wound healing of Sipunculus nudus were studied. An incision,5 mm in length, was cut longitudinally at a site opposite the anus and 10 mm from the introvert. Thehistological study performed at different times showed an involvement of both Type I and Type IIgranulocytes in the process of healing. The former were capable of extracellular digestion and theywere immunoreactive to anti-IL-4, -IL-10 and -epidermal growth factor (EGF) antibodies (Abs); thelatter were involved in the synthesis of connective tissue from 24 h after the incision, thereby causingthe initial closing of the wound. After 70 h, a continuous layer of Type II granulocytes was found on thesides of the wound where the future muscle tissue would be formed; many of these granulocytes hadbeen partially degranulated. It was not possible to establish any existing relationship between thefunctions of Type I granulocytes and their reactivity to anti-IL-4, -IL-10 and -EGF Abs.
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