دورية أكاديمية
The effect of matrices on the gene expression profile of patient-derived head and neck carcinoma cells for in vitro therapy testing
| العنوان: | The effect of matrices on the gene expression profile of patient-derived head and neck carcinoma cells for in vitro therapy testing |
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| المؤلفون: | Aini Hyytiäinen, Katja Korelin, Mervi Toriseva, Tommy Wilkman, Satu Kainulainen, Karri Mesimäki, Johannes Routila, Sami Ventelä, Heikki Irjala, Matthias Nees, Ahmed Al-Samadi, Tuula Salo |
| المصدر: | Cancer Cell International, Vol 23, Iss 1, Pp 1-22 (2023) |
| بيانات النشر: | BMC, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens LCC:Cytology |
| مصطلحات موضوعية: | Head and neck cancer, Patient-derived cells, Gene expression, In vitro 3D, Tumor microenvironment, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282, Cytology, QH573-671 |
| الوصف: | Abstract Objective Head and neck squamous cell carcinoma (HNSCC) is a highly aggressive tumor with a 5-year mortality rate of ~ 50%. New in vitro methods are needed for testing patients’ cancer cell response to anti-cancer treatments. We aimed to investigate how the gene expression of fresh carcinoma tissue samples and freshly digested single cancer cells change after short-term cell culturing on plastic, Matrigel or Myogel. Additionally, we studied the effect of these changes on the cancer cells’ response to anti-cancer treatments. Materials/methods Fresh tissue samples from HNSCC patients were obtained perioperatively and single cells were enzymatically isolated and cultured on either plastic, Matrigel or Myogel. We treated the cultured cells with cisplatin, cetuximab, and irradiation; and performed cell viability measurement. RNA was isolated from fresh tissue samples, freshly isolated single cells and cultured cells, and RNA sequencing transcriptome profiling and gene set enrichment analysis were performed. Results Cancer cells obtained from fresh tissue samples changed their gene expression regardless of the culturing conditions, which may be due to the enzymatic digestion of the tissue. Myogel was more effective than Matrigel at supporting the upregulation of pathways related to cancer cell proliferation and invasion. The impacts of anti-cancer treatments varied between culturing conditions. Conclusions Our study showed the challenge of in vitro cancer drug testing using enzymatic cell digestion. The upregulation of many targeted pathways in the cultured cells may partially explain the common clinical failure of the targeted cancer drugs that pass the in vitro testing. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1475-2867 |
| العلاقة: | https://doaj.org/toc/1475-2867Test |
| DOI: | 10.1186/s12935-023-02982-y |
| الوصول الحر: | https://doaj.org/article/c23305f8303945c3bb4173ddd2f38beaTest |
| رقم الانضمام: | edsdoj.23305f8303945c3bb4173ddd2f38bea |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14752867 |
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| DOI: | 10.1186/s12935-023-02982-y |