دورية أكاديمية
Efficacy and safety of mesenchymal stem cell therapy for ovarian ageing in a mouse model
| العنوان: | Efficacy and safety of mesenchymal stem cell therapy for ovarian ageing in a mouse model |
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| المؤلفون: | Wendi Pei, Lin Fu, Wenhuan Guo, Yibo Wang, Yong Fan, Rui Yang, Rong Li, Jie Qiao, Yang Yu |
| المصدر: | Stem Cell Research & Therapy, Vol 15, Iss 1, Pp 1-16 (2024) |
| بيانات النشر: | BMC, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Medicine (General) LCC:Biochemistry |
| مصطلحات موضوعية: | Mesenchymal stem cell, Ovary, Ageing, Mouse model, Safety, Medicine (General), R5-920, Biochemistry, QD415-436 |
| الوصف: | Abstract Background Ovarian ageing is one of the major issues that impacts female fertility. Mesenchymal stem cell (MSC)-based therapy has made impressive progress in recent years. However, the efficacy and safety of MSCs, as nonautologous components, remain to be further verified. Methods Two common sources of MSCs, umbilical cord-derived MSCs (UC-MSCs) and adipose tissue-derived MSCs (AD-MSCs), were orthotopically transplanted into a mouse model of ovarian ageing to evaluate their therapeutic effects. The safety of the treatment was further evaluated, and RNA sequencing was performed to explore the underlying mechanisms involved. Results After orthotopic transplantation of MSCs into the ovary, the oestrous cycle, ovarian weight, number and proportion of primary follicles, granulosa cell proliferation, and angiogenesis were improved. The effects of AD-MSCs were superior to those of UC-MSCs in several indices, such as post-transplant granulosa cell proliferation, ovarian weight and angiogenesis. Moreover, the tumorigenesis, acute toxicity, immunogenicity and biodistribution of MSCs were evaluated, and both AD-MSCs and UC-MSCs were found to possess high safety profiles. Through RNA sequencing analysis, enhancement of the MAPK cascade was observed, and long-term effects were mainly linked to the activation of immune function. Conclusions Orthotopic transplantation of MSCs displays significant efficacy and high safety for the treatment of ovarian ageing in mice. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1757-6512 29271649 |
| العلاقة: | https://doaj.org/toc/1757-6512Test |
| DOI: | 10.1186/s13287-024-03698-0 |
| الوصول الحر: | https://doaj.org/article/1f8ac20cad814ab8bf29271649f0b3c2Test |
| رقم الانضمام: | edsdoj.1f8ac20cad814ab8bf29271649f0b3c2 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17576512 29271649 |
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| DOI: | 10.1186/s13287-024-03698-0 |