دورية أكاديمية
Rapid Detection and Differentiation of Clonorchis sinensis and Opisthorchis viverrini Using Real-Time PCR and High Resolution Melting Analysis
| العنوان: | Rapid Detection and Differentiation of Clonorchis sinensis and Opisthorchis viverrini Using Real-Time PCR and High Resolution Melting Analysis |
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| المؤلفون: | Xian-Quan Cai, Hai-Qiong Yu, Rong Li, Qiao-Yun Yue, Guo-Hua Liu, Jian-Shan Bai, Yan Deng, De-Yi Qiu, Xing-Quan Zhu |
| المصدر: | The Scientific World Journal, Vol 2014 (2014) |
| بيانات النشر: | Hindawi Limited, 2014. |
| سنة النشر: | 2014 |
| المجموعة: | LCC:Technology LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Technology, Medicine, Science |
| الوصف: | Clonorchis sinensis and Opisthorchis viverrini are both important fish-borne pathogens, causing serious public health problem in Asia. The present study developed an assay integrating real-time PCR and high resolution melting (HRM) analysis for the specific detection and rapid identification of C. sinensis and O. viverrini. Primers targeting COX1 gene were highly specific for these liver flukes, as evidenced by the negative amplification of closely related trematodes. Assays using genomic DNA extracted from the two flukes yielded specific amplification and their identity was confirmed by sequencing, having the accuracy of 100% in reference to conventional methods. The assay was proved to be highly sensitive with a detection limit below 1 pg of purified genomic DNA, 5 EPG, or 1 metacercaria of C. sinensis. Moreover, C. sinensis and O. viverrini were able to be differentiated by their HRM profiles. The method can reduce labor of microscopic examination and the contamination of agarose electrophoresis. Moreover, it can differentiate these two flukes which are difficult to be distinguished using other methods. The established method provides an alternative tool for rapid, simple, and duplex detection of C. sinensis and O. viverrini. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2356-6140 1537-744X |
| العلاقة: | https://doaj.org/toc/2356-6140Test; https://doaj.org/toc/1537-744XTest |
| DOI: | 10.1155/2014/893981 |
| الوصول الحر: | https://doaj.org/article/1d13228e19cf4de4b416dfb0b36ed312Test |
| رقم الانضمام: | edsdoj.1d13228e19cf4de4b416dfb0b36ed312 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23566140 1537744X |
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| DOI: | 10.1155/2014/893981 |