دورية أكاديمية
Genetic screen in Drosophila muscle identifies autophagy-mediated T-tubule remodeling and a Rab2 role in autophagy
| العنوان: | Genetic screen in Drosophila muscle identifies autophagy-mediated T-tubule remodeling and a Rab2 role in autophagy |
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| المؤلفون: | Naonobu Fujita, Wilson Huang, Tzu-han Lin, Jean-Francois Groulx, Steve Jean, Jen Nguyen, Yoshihiko Kuchitsu, Ikuko Koyama-Honda, Noboru Mizushima, Mitsunori Fukuda, Amy A Kiger |
| المصدر: | eLife, Vol 6 (2017) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | T-tubule, autophagy, Rab GTPase, muscle fiber, autophagosome-lysosome fusion, Drosophila metamorphosis, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | Transverse (T)-tubules make-up a specialized network of tubulated muscle cell membranes involved in excitation-contraction coupling for power of contraction. Little is known about how T-tubules maintain highly organized structures and contacts throughout the contractile system despite the ongoing muscle remodeling that occurs with muscle atrophy, damage and aging. We uncovered an essential role for autophagy in T-tubule remodeling with genetic screens of a developmentally regulated remodeling program in Drosophila abdominal muscles. Here, we show that autophagy is both upregulated with and required for progression through T-tubule disassembly stages. Along with known mediators of autophagosome-lysosome fusion, our screens uncovered an unexpected shared role for Rab2 with a broadly conserved function in autophagic clearance. Rab2 localizes to autophagosomes and binds to HOPS complex members, suggesting a direct role in autophagosome tethering/fusion. Together, the high membrane flux with muscle remodeling permits unprecedented analysis both of T-tubule dynamics and fundamental trafficking mechanisms. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| العلاقة: | https://elifesciences.org/articles/23367Test; https://doaj.org/toc/2050-084XTest |
| DOI: | 10.7554/eLife.23367 |
| الوصول الحر: | https://doaj.org/article/1cacc78f0b644c31b7ecb37658e9d198Test |
| رقم الانضمام: | edsdoj.1cacc78f0b644c31b7ecb37658e9d198 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.23367 |