دورية أكاديمية
Changes in Carbohydrate Metabolism in Plasmopara viticola-Infected Grapevine Leaves
| العنوان: | Changes in Carbohydrate Metabolism in Plasmopara viticola-Infected Grapevine Leaves |
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| المؤلفون: | Magdalena Gamm, Marie-Claire Héloir, Richard Bligny, Nathalie Vaillant-Gaveau, Sophie Trouvelot, Gérard Alcaraz, Patrick Frettinger, Christophe Clément, Alain Pugin, David Wendehenne, Marielle Adrian |
| المصدر: | Molecular Plant-Microbe Interactions, Vol 24, Iss 9, Pp 1061-1073 (2011) |
| بيانات النشر: | The American Phytopathological Society, 2011. |
| سنة النشر: | 2011 |
| المجموعة: | LCC:Microbiology LCC:Botany |
| مصطلحات موضوعية: | Microbiology, QR1-502, Botany, QK1-989 |
| الوصف: | The oomycete Plasmopara viticola is responsible for downy mildew, a severe grapevine disease. In infected grapevine leaves, we have observed an abnormal starch accumulation at the end of the dark period, suggesting modifications in starch metabolism. Therefore, several complementary approaches, including transcriptomic analyses, measurements of enzyme activities, and sugar quantification, were performed in order to investigate and to understand the effects of P. viticola infection on leaf starch and—to a larger extent—carbohydrate metabolism. Our results indicate that starch accumulation is associated with an increase in ADP-glucose pyrophosphorylase (AGPase) activity and modifications in the starch degradation pathway, especially an increased α-amylase activity. Together with these alterations in starch metabolism, we have observed an accumulation of hexoses, an increase in invertase activity, and a reduction of photosynthesis, indicating a source-to-sink transition in infected leaf tissue. Additionally, we have measured an accumulation of the disaccharide trehalose correlated to an increased trehalase gene expression and enzyme activity. Altogether, these results highlight a dramatic alteration of carbohydrate metabolism correlated with later stages of P. viticola development in leaves. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1943-7706 0894-0282 |
| العلاقة: | https://doaj.org/toc/0894-0282Test; https://doaj.org/toc/1943-7706Test |
| DOI: | 10.1094/MPMI-02-11-0040 |
| الوصول الحر: | https://doaj.org/article/1283d72815a64f338fefd521b124a0a6Test |
| رقم الانضمام: | edsdoj.1283d72815a64f338fefd521b124a0a6 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19437706 08940282 |
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| DOI: | 10.1094/MPMI-02-11-0040 |