دورية أكاديمية
Understanding the Behaviour of Human Cell Types under Simulated Microgravity Conditions: The Case of Erythrocytes
| العنوان: | Understanding the Behaviour of Human Cell Types under Simulated Microgravity Conditions: The Case of Erythrocytes |
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| المؤلفون: | Cristina Manis, Alessia Manca, Antonio Murgia, Giuseppe Uras, Pierluigi Caboni, Terenzio Congiu, Gavino Faa, Antonella Pantaleo, Giacomo Cao |
| المصدر: | International Journal of Molecular Sciences, Vol 23, Iss 12, p 6876 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | erythrocytes, microgravity, confocal and scanning electron microscopy, oxidative stress, mass spectrometry, lipidomics, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Erythrocytes are highly specialized cells in human body, and their main function is to ensure the gas exchanges, O2 and CO2, within the body. The exposure to microgravity environment leads to several health risks such as those affecting red blood cells. In this work, we investigated the changes that occur in the structure and function of red blood cells under simulated microgravity, compared to terrestrial conditions, at different time points using biochemical and biophysical techniques. Erythrocytes exposed to simulated microgravity showed morphological changes, a constant increase in reactive oxygen species (ROS), a significant reduction in total antioxidant capacity (TAC), a remarkable and constant decrease in total glutathione (GSH) concentration, and an augmentation in malondialdehyde (MDA) at increasing times. Moreover, experiments were performed to evaluate the lipid profile of erythrocyte membranes which showed an upregulation in the following membrane phosphocholines (PC): PC16:0_16:0, PC 33:5, PC18:2_18:2, PC 15:1_20:4 and SM d42:1. Thus, remarkable changes in erythrocyte cytoskeletal architecture and membrane stiffness due to oxidative damage have been found under microgravity conditions, in addition to factors that contribute to the plasticity of the red blood cells (RBCs) including shape, size, cell viscosity and membrane rigidity. This study represents our first investigation into the effects of microgravity on erythrocytes and will be followed by other experiments towards understanding the behaviour of different human cell types in microgravity. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 23126876 1422-0067 1661-6596 |
| العلاقة: | https://www.mdpi.com/1422-0067/23/12/6876Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test |
| DOI: | 10.3390/ijms23126876 |
| الوصول الحر: | https://doaj.org/article/0c1d82dea5c84bfdbc95a828fb771056Test |
| رقم الانضمام: | edsdoj.0c1d82dea5c84bfdbc95a828fb771056 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23126876 14220067 16616596 |
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| DOI: | 10.3390/ijms23126876 |