دورية أكاديمية
Acyl-lipid desaturases and Vipp1 cooperate in cyanobacteria to produce novel omega-3 PUFA-containing glycolipids
| العنوان: | Acyl-lipid desaturases and Vipp1 cooperate in cyanobacteria to produce novel omega-3 PUFA-containing glycolipids |
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| المؤلفون: | Leslie B. Poole, Derek Parsonage, Susan Sergeant, Leslie R. Miller, Jingyun Lee, Cristina M. Furdui, Floyd H. Chilton |
| المصدر: | Biotechnology for Biofuels, Vol 13, Iss 1, Pp 1-15 (2020) |
| بيانات النشر: | BMC, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Fuel LCC:Biotechnology |
| مصطلحات موضوعية: | Omega-3 polyunsaturated fatty acids, Bioengineering, Nutrition, Aquaculture, Cyanobacteria, Leptolyngbya, Fuel, TP315-360, Biotechnology, TP248.13-248.65 |
| الوصف: | Abstract Background Dietary omega-3 (n-3), long chain (LC-, ≥ 20 carbons), polyunsaturated fatty acids (PUFAs) derived largely from marine animal sources protect against inflammatory processes and enhance brain development and function. With the depletion of natural stocks of marine animal sources and an increasing demand for n-3 LC-PUFAs, alternative, sustainable supplies are urgently needed. As a result, n-3 18-carbon and LC-PUFAs are being generated from plant or algal sources, either by engineering new biosynthetic pathways or by augmenting existing systems. Results We utilized an engineered plasmid encoding two cyanobacterial acyl-lipid desaturases (DesB and DesD, encoding Δ15 and Δ6 desaturases, respectively) and “vesicle-inducing protein in plastids” (Vipp1) to induce production of stearidonic acid (SDA, 18:4 n-3) at high levels in three strains of cyanobacteria (10, 17 and 27% of total lipids in Anabaena sp. PCC7120, Synechococcus sp. PCC7002, and Leptolyngbya sp. strain BL0902, respectively). Lipidomic analysis revealed that in addition to SDA, the rare anti-inflammatory n-3 LC-PUFA eicosatetraenoic acid (ETA, 20:4 n-3) was synthesized in these engineered strains, and ~ 99% of SDA and ETA was complexed to bioavailable monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) species. Importantly, novel molecular species containing alpha-linolenic acid (ALA), SDA and/or ETA in both acyl positions of MGDG and DGDG were observed in the engineered Leptolyngbya and Synechococcus strains, suggesting that these could provide a rich source of anti-inflammatory molecules. Conclusions Overall, this technology utilizes solar energy, consumes carbon dioxide, and produces large amounts of nutritionally important n-3 PUFAs and LC-PUFAs. Importantly, it can generate previously undescribed, highly bioavailable, anti-inflammatory galactosyl lipids. This technology could therefore be transformative in protecting ocean fisheries and augmenting the nutritional quality of human and animal food products. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1754-6834 |
| العلاقة: | http://link.springer.com/article/10.1186/s13068-020-01719-7Test; https://doaj.org/toc/1754-6834Test |
| DOI: | 10.1186/s13068-020-01719-7 |
| الوصول الحر: | https://doaj.org/article/007456e8de4942cb80063d40080c78baTest |
| رقم الانضمام: | edsdoj.007456e8de4942cb80063d40080c78ba |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17546834 |
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| DOI: | 10.1186/s13068-020-01719-7 |