دورية أكاديمية
Cytotoxic effects induced by oxidative stress in cultured mammalian cells and protection provided by Hsp27 expression.
| العنوان: | Cytotoxic effects induced by oxidative stress in cultured mammalian cells and protection provided by Hsp27 expression. |
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| المؤلفون: | Arrigo, André-Patrick, Firdaus, Wance J J, Mellier, Grégory, Moulin, Maryline, Paul, Catherine, Diaz-Latoud, Chantal, Kretz-Remy, Carole |
| المساهمون: | Centre de génétique et de physiologie moléculaire et cellulaire (CGPhiMC), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 1046-2023. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2005 |
| المجموعة: | HAL Lyon 1 (University Claude Bernard Lyon 1) |
| مصطلحات موضوعية: | MESH: Animals, MESH: Annexin A5, MESH: Coloring Agents, MESH: Cytochromes c, MESH: DNA, MESH: DNA Damage, MESH: Dimerization, MESH: Disulfides, MESH: Enzyme Activation, MESH: Flow Cytometry, MESH: Fluoresceins, MESH: Fluorescent Antibody Technique, Indirect, MESH: Antioxidants, MESH: Genetic Vectors, MESH: Gentian Violet, MESH: Glutathione, MESH: Heat-Shock Proteins, MESH: Hela Cells, MESH: Humans, MESH: Immunoblotting, MESH: Iron, MESH: Lipid Peroxidation, MESH: Mice, MESH: Apoptosis, MESH: Mitochondria, MESH: NIH 3T3 Cells, MESH: Neoplasm Proteins, MESH: Nitric Oxide, MESH: Oligonucleotides |
| الوصف: | There is currently great interest in the development of methods to analyze intracellular redox state and the cellular damages generated by oxidative stress. General methods for analyzing reactive oxygen species and glutathione level are presented together with more recently developed protocols to analyze the consequences of oxidative stress on the oxidation of macromolecules. Finally, techniques to study modalities of constitutive expression of Hsp27 in mammalian cells are considered as well as methods used to determine the protective activity of this small heat shock protein against the deleterious effects induced by oxidative stress. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/15649839; hal-00193428; https://hal.science/hal-00193428Test; PUBMED: 15649839 |
| DOI: | 10.1016/j.ymeth.2004.08.003 |
| الإتاحة: | https://doi.org/10.1016/j.ymeth.2004.08.003Test https://hal.science/hal-00193428Test |
| رقم الانضمام: | edsbas.FDF6908F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.ymeth.2004.08.003 |
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