دورية أكاديمية
Visualization of individual cell division history in complex tissues using iCOUNT
| العنوان: | Visualization of individual cell division history in complex tissues using iCOUNT |
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| المؤلفون: | Denoth-Lippuner, Annina, Jaeger, Baptiste N, Liang, Tong, Royall, Lars N, Chie, Stefanie E, Buthey, Kilian, Machado, Diana, Korobeynyk, Vladislav I, Kruse, Merit, Munz, Clara M, Gerbaulet, Alexander, Simons, Benjamin D, Jessberger, Sebastian |
| المصدر: | Denoth-Lippuner, Annina; Jaeger, Baptiste N; Liang, Tong; Royall, Lars N; Chie, Stefanie E; Buthey, Kilian; Machado, Diana; Korobeynyk, Vladislav I; Kruse, Merit; Munz, Clara M; Gerbaulet, Alexander; Simons, Benjamin D; Jessberger, Sebastian (2021). Visualization of individual cell division history in complex tissues using iCOUNT. Cell Stem Cell, 28(11):2020-2034.e12. |
| بيانات النشر: | Cell Press (Elsevier) |
| سنة النشر: | 2021 |
| المجموعة: | University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
| مصطلحات موضوعية: | Brain Research Institute, 570 Life sciences, biology, 610 Medicine & health |
| الوصف: | The division potential of individual stem cells and the molecular consequences of successive rounds of proliferation remain largely unknown. Here, we developed an inducible cell division counter (iCOUNT) that reports cell division events in human and mouse tissues in vitro and in vivo. Analyzing cell division histories of neural stem/progenitor cells (NSPCs) in the developing and adult brain, we show that iCOUNT can provide novel insights into stem cell behavior. Further, we use single-cell RNA sequencing (scRNA-seq) of iCOUNT-labeled NSPCs and their progenies from the developing mouse cortex and forebrain-regionalized human organoids to identify functionally relevant molecular pathways that are commonly regulated between mouse and human cells, depending on individual cell division histories. Thus, we developed a tool to characterize the molecular consequences of repeated cell divisions of stem cells that allows an analysis of the cellular principles underlying tissue formation, homeostasis, and repair. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1875-9777 |
| العلاقة: | https://www.zora.uzh.ch/id/eprint/213995/1/PIIS1934590921003490.pdfTest; info:pmid/34525348; urn:issn:1875-9777 |
| DOI: | 10.5167/uzh-213995 |
| DOI: | 10.1016/j.stem.2021.08.012 |
| الإتاحة: | https://doi.org/10.5167/uzh-21399510.1016/j.stem.2021.08.012Test https://www.zora.uzh.ch/id/eprint/213995Test/ https://www.zora.uzh.ch/id/eprint/213995/1/PIIS1934590921003490.pdfTest |
| حقوق: | info:eu-repo/semantics/openAccess ; Creative Commons: Attribution 4.0 International (CC BY 4.0) ; http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.FDE134AD |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 18759777 |
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| DOI: | 10.5167/uzh-213995 |