دورية أكاديمية
S-layer from Lactobacillus brevis modulates antigen-presenting cell functions via the Mincle-Syk-Card9 axis
| العنوان: | S-layer from Lactobacillus brevis modulates antigen-presenting cell functions via the Mincle-Syk-Card9 axis |
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| المؤلفون: | Prado Acosta, Mariano, Goyette-Desjardins, Guillaume, Scheffel, Jörg, Dudeck, Anne, Ruland, Jürgen, Lepenies, Bernd |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | article, ddc:630, Hochschulbibliographie allgemein, Verzeichnis wissenschaftlicher Veröffentlichungen, Antigen-Presenting Cells, CD4-Positive T-Lymphocytes, Bone Marrow Cells, Animals, Mice, Knockout, Membrane Glycoproteins, Lectins, C-Type, Membrane Proteins, Cytokines, Signal Transduction, Lactobacillus brevis, CARD Signaling Adaptor Proteins, Syk Kinase, s-layer, Antigen presenting cell, Mincle, Card9, Syk (Spleen Tyrosine Kinase) |
| الوقت: | 2021 |
| الوصف: | C-type lectin receptors (CLRs) are pattern recognition receptors that are crucial in the innate immune response. The gastrointestinal tract contributes significantly to the maintenance of immune homeostasis; it is the shelter for billions of microorganisms including many genera of Lactobacillus sp. Previously, it was shown that host-CLR interactions with gut microbiota play a crucial role in this context. The Macrophage-inducible C-type lectin (Mincle) is a Syk-coupled CLR that contributes to sensing of mucosa-associated commensals. In this study, we identified Mincle as a receptor for the Surface (S)-layer of the probiotic bacteria Lactobacillus brevis modulating GM-CSF bone marrow-derived cells (BMDCs) functions. We found that the S-layer/Mincle interaction led to a balanced cytokine response in BMDCs by triggering the release of both pro- and anti-inflammatory cytokines. In contrast, BMDCs derived from Mincle-/-, CARD9-/- or conditional Syk-/- mice failed to maintain this balance, thus leading to an increased production of the pro-inflammatory cytokines TNF and IL-6, whereas the levels of the anti-inflammatory cytokines IL-10 and TGF-β were markedly decreased. Importantly, this was accompanied by an altered CD4+ T cell priming capacity of Mincle-/- BMDCs resulting in an increased CD4+ T cell IFN-γ production upon stimulation with L. brevis S-layer. Our results contribute to the understanding of how commensal bacteria regulate antigen-presenting cell (APC) functions and highlight the importance of the Mincle/Syk/Card9 axis in APCs as a key factor in host-microbiota interactions. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | Frontiers in immunology -- Front Immunol -- http://www.frontiersin.org/immunologyTest -- https://www.ncbi.nlm.nih.gov/pmc/journals/1754Test/ -- http://www.bibliothek.uni-regensburg.de/ezeit/?2606827Test -- 2606827-8 -- 1664-3224; https://doi.org/10.3389/fimmu.2021.602067Test; https://elib.tiho-hannover.de/receive/tiho_mods_00005083Test; https://elib.tiho-hannover.de/servlets/MCRFileNodeServlet/tiho_derivate_00001167/fimmu-12-602067.pdfTest; https://europepmc.org/articles/PMC7957004Test; https://www.frontiersin.org/articles/10.3389/fimmu.2021.602067/fullTest |
| DOI: | 10.3389/fimmu.2021.602067 |
| DOI: | 10.3389/fimmu.2021.602067/full |
| الإتاحة: | https://doi.org/10.3389/fimmu.2021.602067Test https://elib.tiho-hannover.de/receive/tiho_mods_00005083Test https://elib.tiho-hannover.de/servlets/MCRFileNodeServlet/tiho_derivate_00001167/fimmu-12-602067.pdfTest https://europepmc.org/articles/PMC7957004Test |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test/ ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.FD4AFF2B |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fimmu.2021.602067 |
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