التفاصيل البيبلوغرافية
| العنوان: |
Analyzing BoNT-induced urinary retention in vivo. |
| المؤلفون: |
Hatim Thaker (7928519), Jie Zhang (64655), Shin-Ichiro Miyashita (6184568), Vivian Cristofaro (586878), SunHyun Park (11576924), Ali Hashemi Gheinani (11619876), Maryrose P. Sullivan (11576930), Rosalyn M. Adam (11576933), Min Dong (123378) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Microbiology, Cell Biology, Genetics, Molecular Biology, Neuroscience, Physiology, Pharmacology, Biotechnology, Evolutionary Biology, Immunology, Cancer, Infectious Diseases, Chemical Sciences not elsewhere classified, wild type mice, target neurons determines, skeletal neuromuscular junctions, potent toxins known, dominant toxin receptor, bonts &# 8217, analyzing bont action, dominant receptor, injecting toxins, wide range, two sets, therapeutic efficacy, overactive bladder, leg muscle, findings establish |
| الوصف: |
A. Schematic illustration of the bladder injection model. Mice were anesthetized with isoflurane and a low midline laparotomy was made. The bladder was exteriorized and decompressed of urine to thicken the bladder wall. BoNT/B was injected into the lateral walls of the bladder bilaterally. B. Mice injected with BoNT/B were subjected to 4-hour nighttime voiding spot assay every 24 hours for 4 days. Representative images for urine spots (black areas) are shown. C. Voiding spots quantified into total void areas (inch 2 ) demonstrate similar pre-injection urine volumes across WT, Syt1 M3 , and Syt2 M3 mice (n = 5 for each group). All groups showed a decline in voided volumes in sham controls that went through the surgery and injection procedures without toxins, likely from surgical manipulation of the bladder and the use of post-operative pain control narcotics. n = 3 for each group. D. At 24–48 hours post-injection, WT and Syt2 KI mice had significantly diminished voided volumes compared to Syt1 KI mice, with progressive recovery over 96 hours. *P<0.05, **P<0.005, ns = not significant. E. Number of voiding spots per 4-hour nighttime evaluation showed no difference across genotypes, and across pre- and post-injection time points, demonstrating that voiding frequency is unchanged across groups. ns = not significant. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/figure/Analyzing_BoNT-induced_urinary_retention_in_vivo_/16827728Test |
| DOI: |
10.1371/journal.ppat.1009994.g006 |
| الإتاحة: |
https://doi.org/10.1371/journal.ppat.1009994.g006Test |
| حقوق: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.FD26A2FD |
| قاعدة البيانات: |
BASE |
