دورية أكاديمية
Dendritic spine loss in epileptogenic Type II focal cortical dysplasia: Role of enhanced classical complement pathway activation
| العنوان: | Dendritic spine loss in epileptogenic Type II focal cortical dysplasia: Role of enhanced classical complement pathway activation |
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| المؤلفون: | Rossini, Laura, De Santis, Dalia, Cecchini, Erica, Cagnoli, Cinzia, Maderna, Emanuela, Cartelli, Daniele, Morgan, Bryan Paul, Torvell, Megan, Spreafico, Roberto, di Giacomo, Roberta, Tassi, Laura, de Curtis, Marco, Garbelli, Rita |
| بيانات النشر: | Wiley Open Access |
| سنة النشر: | 2022 |
| المجموعة: | Cardiff University: ORCA (Online Research @ Cardiff) |
| الوصف: | Dendritic spines are the postsynaptic sites for most excitatory glutamatergic synapses. We previously demonstrated a severe spine loss and synaptic reorganization in human neocortices presenting Type II focal cortical dysplasia (FCD), a developmental malformation and frequent cause of drug‐resistant focal epilepsy. We extend the findings, investigating the potential role of complement components C1q and C3 in synaptic pruning imbalance. Data from Type II FCD were compared with those obtained in focal epilepsies with different etiologies. Neocortical tissues were collected from 20 subjects, mainly adults with a mean age at surgery of 31 years, admitted to epilepsy surgery with a neuropathological diagnosis of: cryptogenic, temporal lobe epilepsy with hippocampal sclerosis, and Type IIa/b FCD. Dendritic spine density quantitation, evaluated in a previous paper using Golgi impregnation, was available in a subgroup. Immunohistochemistry, in situ hybridization, electron microscopy, and organotypic cultures were utilized to study complement/microglial activation patterns. FCD Type II samples presenting dendritic spine loss were characterized by an activation of the classical complement pathway and microglial reactivity. In the same samples, a close relationship between microglial cells and dendritic segments/synapses was found. These features were consistently observed in Type IIb FCD and in 1 of 3 Type IIa cases. In other patient groups and in perilesional areas outside the dysplasia, not presenting spine loss, these features were not observed. In vitro treatment with complement proteins of organotypic slices of cortical tissue with no sign of FCD induced a reduction in dendritic spine density. These data suggest that dysregulation of the complement system plays a role in microglia‐mediated spine loss. This mechanism, known to be involved in the removal of redundant synapses during development, is likely reactivated in Type II FCD, particularly in Type IIb; local treatment with anticomplement drugs could in principle ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://orca.cardiff.ac.uk/id/eprint/155337/1/bpa.13141.pdfTest; Rossini, Laura, De Santis, Dalia, Cecchini, Erica, Cagnoli, Cinzia, Maderna, Emanuela, Cartelli, Daniele, Morgan, Bryan Paul, Torvell, Megan https://orca.cardiff.ac.uk/view/cardiffauthors/A23930503.htmlTest, Spreafico, Roberto, di Giacomo, Roberta, Tassi, Laura, de Curtis, Marco and Garbelli, Rita 2022. Dendritic spine loss in epileptogenic Type II focal cortical dysplasia: Role of enhanced classical complement pathway activation. Brain Pathology , e13141. 10.1111/bpa.13141 https://doi.org/10.1111/bpa.13141Test file https://orca.cardiff.ac.uk/id/eprint/155337/1/bpa.13141.pdfTest |
| DOI: | 10.1111/bpa.13141 |
| الإتاحة: | https://doi.org/10.1111/bpa.13141Test https://orca.cardiff.ac.uk/id/eprint/155337Test/ https://orca.cardiff.ac.uk/id/eprint/155337/1/bpa.13141.pdfTest |
| حقوق: | cc_by_nc_nd_4_0 |
| رقم الانضمام: | edsbas.FC5DC951 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/bpa.13141 |
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