دورية أكاديمية
Radiosensitizing effect of polysaccharide in Schisandrae chinensis (Turcz.) Baill. Fruit on ovarian cancer SKOV3 cells
| العنوان: | Radiosensitizing effect of polysaccharide in Schisandrae chinensis (Turcz.) Baill. Fruit on ovarian cancer SKOV3 cells |
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| المؤلفون: | Wang, Cai-Xia, Wan, Dan, Qiao, Shi-Cong, Xia, Min, Duan, Ya-Ting |
| المصدر: | Tropical Journal of Pharmaceutical Research; Vol. 20 No. 3 (2021); 633-638 ; 1596-9827 ; 1596-5996 |
| بيانات النشر: | Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria |
| سنة النشر: | 2022 |
| المجموعة: | AJOL - African Journals Online |
| مصطلحات موضوعية: | Radiosensitizing, Polysaccharide, Schisandrae chinensis fructus, β-Irradiation, Ovarian cancer, SKOV3 cell line |
| الوصف: | Purpose: To investigate the radiosensitizing effect of polysaccharide from Schisandrae Chinensis fruit (SCFP) on ovarian cancer SKOV3 cell line. Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate SKOV3 cell viability. Caspase-3, caspase-9, Bax, and Bcl-2 levels in SKOV3 cells were assessed by enzyme-linked immunosorbent assay (ELISA). Furthermore, mRNA expression of caspase-3, caspase-9, Bax and Bcl-2 in SKOV3 cells were determined using quantitative real-time polymerase chain reaction (RT-qPCR) assay. Results: β-Irradiation (10 Gy) did not show obvious inhibitory effects on SKOV3 cell proliferation. However, SCFP at a dose of 1.25, 2.5 and 5 mg/mL significantly enhanced the radiosensitivity of SKOV3 cells to β-irradiation (p < 0.01). Furthermore, SCFP at concentrations of 1.25, 2.5 and 5 mg/mL significantly increased the levels of caspase-3, caspase-9 and Bax in SKOV3 cells, but decreased that of Bcl-2 (p < 0.01). In addition, SCFP combined with β-irradiation up-regulated mRNA expression of caspase-3, caspase-9 and Bax (p < 0.01), but down-regulated that of Bcl-2 in SKOV3 cells (p < 0.01). Conclusion: SCFP significantly enhances the sensitivity of nasopharyngeal carcinoma SKOV3 cells to β-irradiation. The possible mechanism of sensitization might be correlated to the induction of mitochondria-mediated apoptosis. This research provides a scientific basis for the development of SCFP for the treatment of ovarian cancer. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://www.ajol.info/index.php/tjpr/article/view/220171/207759Test; https://www.ajol.info/index.php/tjpr/article/view/220171Test |
| DOI: | 10.4314/tjpr.v20i3.28 |
| الإتاحة: | https://doi.org/10.4314/tjpr.v20i3.28Test https://www.ajol.info/index.php/tjpr/article/view/220171Test |
| رقم الانضمام: | edsbas.FC2ADD02 |
| قاعدة البيانات: | BASE |
| DOI: | 10.4314/tjpr.v20i3.28 |
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