التفاصيل البيبلوغرافية
| العنوان: |
紫草萘醌类化合物对乳酸脱氢酶和乙醇脱氢酶的共价修饰作用 |
| المؤلفون: |
段志芳, 黄志纾, 马 林, 车镇涛, 古练权 |
| سنة النشر: |
2001 |
| المجموعة: |
The Hong Kong University of Science and Technology: HKUST Institutional Repository |
| مصطلحات موضوعية: |
紫草萘醌類化合物, 共價修飾作用, 乳酸脫氫酶, 乙醇脫氫酶, alkannin derivatives, covalent modification, LDHase, ADHase |
| الوصف: |
為研究紫草萘醌類化合物的細胞毒性作用機制 ,從新疆軟紫草根中分離出四種紫草萘醌類化合物 β ,β 二甲基丙烯酰阿卡寧 (1) ,乙酰阿卡寧 (2 ) ,β 乙酰氧基異戊酰阿卡寧 (3)和阿卡寧 (4)。研究了四種天然紫草萘醌類化合物對乳酸脫氫酶和乙醇脫氫酶的共價修飾作用。酶活力測定結果表明 ,這四種紫草萘醌類化合物對兩種脫氫酶都具有不同程度的抑制作用 ;酶分子中游離氨基和巰基修飾率的測定結果表明 ,紫草萘醌類化合物對兩種酶的抑制作用主要是通過與酶分子中的巰基共價結合產生的。 Covalent modification of four isolated alkannin derivatives ( alkannin, acetylalkannin, β, β-dimethylacrylalkannin and β-acetoxyisovalerylalkannin, respectively) on rabbit muscle latate dehydrogenase ( LDHase) and yeast Alcohol Dehydrogenase ( ADHase) has been studied .The quinone concentration curves show that the two enzymes are inhibited to different extents by the four alkannin derivatives .Assays of free amino groups and thiol groups in the enzymes indicate that the catalytic activities of LDHase and ADHase are mainly inhibited by covalent reaction of alkannin derivatives with thiol groups in enzymes .These results could be useful for investigating the biological mechanism of alkannin derivatives′cytotoxicity . |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
Chinese |
| تدمد: |
0253-2786 |
| العلاقة: |
http://repository.ust.hk/ir/Record/1783.1-107462Test; 有機化學=Chinese Journal of Organic Chemistry, v. 21, (9), 2001, p. 668-671 |
| الإتاحة: |
http://repository.ust.hk/ir/Record/1783.1-107462Test |
| رقم الانضمام: |
edsbas.FBF68043 |
| قاعدة البيانات: |
BASE |
