كتاب
DamID analysis of nuclear organization in Caenorhabditis elegans
| العنوان: | DamID analysis of nuclear organization in Caenorhabditis elegans |
|---|---|
| المؤلفون: | Gómez-Saldívar, Georgina, Meister, Peter, Askjaer, Peter |
| المساهمون: | Ministerio de Economía y Competitividad (España), European Commission, Swiss National Science Foundation, Consejo Superior de Investigaciones Científicas (España), University of Bern, Swiss Foundation for Research on Muscle Diseases |
| بيانات النشر: | Springer Nature |
| سنة النشر: | 2016 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Nuclear organization, Nuclear envelope, Laminopathies, DamID, Nuclear lamina, Caenorhabditis elegans, Chromatin |
| الوصف: | The development of genomics and next generation sequencing platforms has dramatically improved our insight into chromatin structure and organization and its fine interplay with gene expression. The nuclear envelope has emerged as a key component in nuclear organization via extensive contacts between the genome and numerous proteins at the nuclear periphery. These contacts may have profound effects on gene expression as well as cell proliferation and differentiation. Indeed, their perturbations are associated with several human pathologies known as laminopathies or nuclear envelopathies. However, due to their dynamic behavior the contacts between nuclear envelope proteins and chromatin are challenging to identify, in particular in intact tissues. Here, we propose the DamID technique as an attractive method to globally characterize chromatin organization in the popular model organism Caenorhabditis elegans. DamID is based on the in vivo expression of a chromatin-associated protein of interest fused to the Escherichia coli DNA adenine methyltransferase, which produces unique identification tags at binding site in the genome. This marking is simple, highly specific and can be mapped by sensitive enzymatic and next generation sequencing approaches. ; We gratefully acknowledge funding from the Spanish Ministry of Economy and Competitiveness (BFU2013-42709P) and the European Regional Development Fund to P.A. The Meister laboratory is funded by the Swiss National Foundation (SNF Assistant Professor grant PP00P3_133744), the Swiss Foundation for Muscle Diseases Research, and the University of Bern. G.G.-S. holds a CSIC-JAE Fellowship (JAEPre_2010_00384) and received support from EMBO (ASTF-447-2014) and EC COST Action BM1408 GENiE. ; Peer Reviewed |
| نوع الوثيقة: | book part |
| اللغة: | unknown |
| ردمك: | 978-1-4939-3528-4 1-4939-3528-3 |
| تدمد: | 27147053 |
| العلاقة: | #PLACEHOLDER_PARENT_METADATA_VALUE#; info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2013-42709-P; Methods in Molecular Biology 1411; Sí; The Nuclear Envelope. Methods and Protocols: 341-358 (2016); http://hdl.handle.net/10261/163250Test; http://dx.doi.org/10.13039/501100000780Test; http://dx.doi.org/10.13039/501100003339Test; http://dx.doi.org/10.13039/100009068Test; http://dx.doi.org/10.13039/501100003329Test |
| DOI: | 10.1007/978-1-4939-3530-7_22 |
| DOI: | 10.13039/501100000780 |
| DOI: | 10.13039/501100003339 |
| DOI: | 10.13039/100009068 |
| DOI: | 10.13039/501100003329 |
| الإتاحة: | https://doi.org/10.1007/978-1-4939-3530-7_22Test https://doi.org/10.13039/501100000780Test https://doi.org/10.13039/501100003339Test https://doi.org/10.13039/100009068Test https://doi.org/10.13039/501100003329Test http://hdl.handle.net/10261/163250Test |
| حقوق: | none |
| رقم الانضمام: | edsbas.FB22151E |
| قاعدة البيانات: | BASE |
| ردمك: | 9781493935284 1493935283 |
|---|---|
| تدمد: | 27147053 |
| DOI: | 10.1007/978-1-4939-3530-7_22 |