دورية أكاديمية
The IGHV1-69/IGHJ3 recombinations of unmutated CLL are distinct from those of normal B cells
| العنوان: | The IGHV1-69/IGHJ3 recombinations of unmutated CLL are distinct from those of normal B cells |
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| المؤلفون: | Forconi F, Potter KN, Sozzi E, Henderson I, Cencini E, Bomben R, Gattei V, Packham G, Stevenson F.K., ROSSI, Davide, GAIDANO, Gianluca |
| المساهمون: | Forconi, F, Potter, Kn, Sozzi, E, Henderson, I, Cencini, E, Rossi, Davide, Bomben, R, Gattei, V, Gaidano, Gianluca, Packham, G, Stevenson, F. K. |
| سنة النشر: | 2012 |
| المجموعة: | Università degli Studi del Piemonte Orientale: CINECA IRIS |
| الوصف: | IGHV1-69/51p1 is expressed by ∼ 30% of unmutated chronic lymphocytic leukemia (U-CLL) and combines with selected IGHD and IGHJ genes generating stereotypes if HCDR3 amino acid homology is > 60%. We had previously revealed stereotypic IGHV1-69/IGHJ6 rearrangements in normal naive B cells, thereby identifying potential counterparts of U-CLL. A different stereotypic IGHV1-69/IGHD3-16(RF2)/IGHJ3 rearrangement carrying the CAR(GGx)YD motif in the N1-region, recurrent in 6% IGHV1-69+ve CLL, is exceptionally sequence restricted, strongly suggestive of shared antigen recognition. We have now analyzed IGHV1-69/IGHJ3 rearrangements in circulating B cells of healthy individuals using several PCR-based approaches with IGHV1-69/IGHJ3 CLL sequences for reference. Stereotypes were found, but all were distinct from CLL. Remarkably, even a highly sensitive semi-nested PCR, specific for the CLL-expressed IGHV1-69/IGHD3-16(RF2)/IGHJ3 stereotype, failed to identify the CAR(GGx)YD sequence, although similar motifs were found. These highly specific B cells are not apparent in the accessible normal repertoire and may expand in response to rarely expressed antigens important in the pathogenesis of CLL. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/22234701; info:eu-repo/semantics/altIdentifier/wos/WOS:000300949500021; volume:119; issue:9; firstpage:2106; lastpage:2109; numberofpages:4; journal:BLOOD; http://hdl.handle.net/11579/31366Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84857711341; http://bloodjournal.hematologylibrary.org/content/119/9/2106.longTest |
| DOI: | 10.1182/blood-2011-08-375501 |
| الإتاحة: | https://doi.org/10.1182/blood-2011-08-375501Test http://hdl.handle.net/11579/31366Test http://bloodjournal.hematologylibrary.org/content/119/9/2106.longTest |
| حقوق: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.F9C8A229 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1182/blood-2011-08-375501 |
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