التفاصيل البيبلوغرافية
| العنوان: |
A protein phosphorylation module patterns the Bacillus subtilis spore outer coat |
| المؤلفون: |
Freitas, Carolina, Plannic, Jarnaja, Isticato, Rachele, Pelosi, Assunta, Zilhão, Rita, Serrano, Mónica, Baccigalupi, Loredana, Ricca, Ezio, Elsholz, Alexander K. W., Losick, Richard, O. Henriques, Adriano |
| المساهمون: |
Fundação para a Ciência e a Tecnologia, National Institutes of Health |
| المصدر: |
Molecular Microbiology ; volume 114, issue 6, page 934-951 ; ISSN 0950-382X 1365-2958 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2020 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Assembly of the Bacillus subtilis spore coat involves over 80 proteins which self‐organize into a basal layer, a lamellar inner coat, a striated electrodense outer coat and a more external crust. CotB is an abundant component of the outer coat. The C‐terminal moiety of CotB, SKR B , formed by serine‐rich repeats, is polyphosphorylated by the Ser/Thr kinase CotH. We show that another coat protein, CotG, with a central serine‐repeat region, SKR G , interacts with the C‐terminal moiety of CotB and promotes its phosphorylation by CotH in vivo and in a heterologous system. CotG itself is phosphorylated by CotH but phosphorylation is enhanced in the absence of CotB. Spores of a strain producing an inactive form of CotH, like those formed by a cotG deletion mutant, lack the pattern of electrondense outer coat striations, but retain the crust. In contrast, deletion of the SKR B region, has no major impact on outer coat structure. Thus, phosphorylation of CotG by CotH is a key factor establishing the structure of the outer coat. The presence of the cotB / cotH / cotG cluster in several species closely related to B. subtilis hints at the importance of this protein phosphorylation module in the morphogenesis of the spore surface layers. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1111/mmi.14562 |
| الإتاحة: |
https://doi.org/10.1111/mmi.14562Test |
| حقوق: |
http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.F9BFF9C9 |
| قاعدة البيانات: |
BASE |
