دورية أكاديمية
The ribosome-associated chaperone Zuo1 controls translation upon TORC1 inhibition
| العنوان: | The ribosome-associated chaperone Zuo1 controls translation upon TORC1 inhibition |
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| المؤلفون: | Black, Ailsa, Williams, Thomas D., Soubigou, Flavie, Joshua, Ifeoluwapo M., Zhou, Houjiang, Lamoliatte, Frederic, Rousseau, Adrien |
| المصدر: | Black , A , Williams , T D , Soubigou , F , Joshua , I M , Zhou , H , Lamoliatte , F & Rousseau , A 2023 , ' The ribosome-associated chaperone Zuo1 controls translation upon TORC1 inhibition ' , EMBO Journal , vol. 42 , no. 24 , e113240 . https://doi.org/10.15252/embj.2022113240Test |
| سنة النشر: | 2023 |
| المجموعة: | Discovery - University of Dundee Online Publications |
| مصطلحات موضوعية: | proteostasis, ribosome-associated chaperones, stress, TORC1, translation, /dk/atira/pure/subjectarea/asjc/2800/2800, name=General Neuroscience, /dk/atira/pure/subjectarea/asjc/1300/1312, name=Molecular Biology, /dk/atira/pure/subjectarea/asjc/1300/1300, name=General Biochemistry,Genetics and Molecular Biology, /dk/atira/pure/subjectarea/asjc/2400/2400, name=General Immunology and Microbiology |
| الوصف: | Protein requirements of eukaryotic cells are ensured by proteostasis, which is mediated by tight control of TORC1 activity. Upon TORC1 inhibition, protein degradation is increased and protein synthesis is reduced through inhibition of translation initiation to maintain cell viability. Here, we show that the ribosome-associated complex (RAC)/Ssb chaperone system, composed of the HSP70 chaperone Ssb and its HSP40 co-chaperone Zuo1, is required to maintain proteostasis and cell viability under TORC1 inhibition in Saccharomyces cerevisiae. In the absence of Zuo1, translation does not decrease in response to the loss of TORC1 activity. A functional interaction between Zuo1 and Ssb is required for proper translational control and proteostasis maintenance upon TORC1 inhibition. Furthermore, we have shown that the rapid degradation of eIF4G following TORC1 inhibition is mediated by autophagy and is prevented in zuo1Δ cells, contributing to decreased survival in these conditions. We found that autophagy is defective in zuo1Δ cells, which impedes eIF4G degradation upon TORC1 inhibition. Our findings identify an essential role for RAC/Ssb in regulating translation in response to changes in TORC1 signalling. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://discovery.dundee.ac.uk/en/publications/2a8989b0-6344-4eed-9fe2-17cddcf9055fTest |
| DOI: | 10.15252/embj.2022113240 |
| الإتاحة: | https://doi.org/10.15252/embj.2022113240Test https://discovery.dundee.ac.uk/en/publications/2a8989b0-6344-4eed-9fe2-17cddcf9055fTest https://discovery.dundee.ac.uk/ws/files/114005993/The_EMBO_Journal_-_2023_-_Black_-_The_ribosome_associated_chaperone_Zuo1_controls_translation_upon_TORC1_inhibition.pdfTest http://www.scopus.com/inward/record.url?scp=85177205775&partnerID=8YFLogxKTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.F99705A9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.15252/embj.2022113240 |
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