دورية أكاديمية
Association between serum 25-hydroxyvitamin D and global DNA methylation in visceral adipose tissue from colorectal cancer patients
| العنوان: | Association between serum 25-hydroxyvitamin D and global DNA methylation in visceral adipose tissue from colorectal cancer patients |
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| المؤلفون: | Castellano-Castillo, Daniel, Morcillo, Sonsoles, Crujeiras, Ana B., Sánchez-Alcoholado, Lidia, Clemente-Postigo, Mercedes, Torres, Esperanza, Tinahones, Francisco José, Macias-Gonzalez, Manuel |
| المساهمون: | Castellano-Castillo,D, Morcillo,S, Sánchez-Alcoholado,L, Clemente-Postigo,M, Tinahones,FJ, Macias-Gonzalez,M Unidad de Gestión Clínica Endocrinología y Nutrición. Instituto de Investigación Biomédica de Málaga (IBIMA), Complejo Hospitalario de Málaga (Virgen de la Victoria) Málaga (Spain). CIBER Fisiopatología de la Obesidad y Nutrición (CB06/03), Málaga, Spain. Crujeiras,AB Epigenomics in Endocrinology and Nutrition Group. Instituto de Investigacion Sanitaria (IDIS), Complejo Hospitalario Universitario de Santiago (CHUS) and CIBER Fisiopatologia de la Obesidad y Nutricion (CIBERobn), Málaga, Spain. Torres,E Unidad de Gestión Clínica de Oncología Intercentros Hospital Universitario Virgen de la Victoria, Málaga, Spain. |
| بيانات النشر: | BioMed Central (BMC) |
| سنة النشر: | 2019 |
| المجموعة: | REPISALUD (REPositorio Institucional en SALUD del Instituto de Salud Carlos III - ISCIII) |
| مصطلحات موضوعية: | Colorectal cancer, Global DNA methylation, Obesity, Epigenetic, Vitamin D, Neoplasias colorrectales, Metilación de ADN, Obesidad, Epigénesis genética, Epigenómica, Vitamina D, Colorectal Neoplasms, DNA Methylation, Sequence Analysis, DNA, Nucleotide Elements, Enzyme-Linked Immunosorbent Assay|Aged, Male, Female, Humans, Intra-Abdominal Fat |
| الوصف: | Background: Visceral adipose tissue (VAT) has been identified as the essential fat depot for pathogenetic theories that associateobesity and colon cancer. LINE-1 hypomethylation has been mostly detected in tumor colon tissue, but less is known about the epigenetic pattern in surrounding tissues. The aim was to analyze for the first time the potential relationship between serum vitamin D, obesity and global methylation (LINE-1) in the visceral adipose tissue (VAT) from patients with and without colorectal cancer. Methods: A total of 55 patients with colorectal cancer and 35 control subjects participated in the study. LINE-1 DNA methylation in VAT was measured by pyrosequencing. Serum 25(OH)D levels were determined by ELISA. Results: Cancer patients had lower levels of LINE-1 methylation in VAT compared with the control group. In the subjects with colorectal cancer, LINE-1 DNA methylation levels were associated positively with vitamin D levels (r = 0,463; p < 0.001) and negatively with BMI (r = − 0.334, p = 0.01) and HOMA insulin resistance index (r = − 0.348, p = 0.01). Serum vitamin D was the main variable explaining the LINE-1% variance in the cancer group (β = 0.460, p < 0.001). In a multivariate analysis, subjects with higher LINE-1 methylation values had lower risk of developing colorectal cancer (OR = 0.53; IC95% =0.28–0.99) compared with the control group. Conclusions: We showed for the first time an association between LINE-1 DNA methylation in VAT and vitamin D levels in subjects with colorectal cancer, highlighting the importance of VAT from cancer patients, which could be modified epigenetically compared to healthy subjects. ; This study was supported by “Centros de Investigación En Red” (CIBER, CB06/03/0018) of the “Instituto de Salud Carlos III” (ISCIII), and grants from ISCIII (PI11/01661) and from Consejeria de Innovacion, Ciencia y Empresa de la Junta de Andalucia (PI11-CTS-8181). Co financed by the European Regional Development Fund (FEDER). |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1471-2407 |
| العلاقة: | https://bmccancer.biomedcentral.com/articles/10.1186/s12885-018-5226-4Test; http://hdl.handle.net/10668/3182Test; http://hdl.handle.net/20.500.12105/17811Test; BMC Cancer |
| DOI: | 10.1186/s12885-018-5226-4 |
| الإتاحة: | https://doi.org/20.500.12105/17811Test https://doi.org/10.1186/s12885-018-5226-4Test http://hdl.handle.net/10668/3182Test https://hdl.handle.net/20.500.12105/17811Test |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test/ ; Attribution 4.0 International ; open access |
| رقم الانضمام: | edsbas.F8FFA73F |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14712407 |
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| DOI: | 10.1186/s12885-018-5226-4 |