دورية أكاديمية
Morphological remodeling of Coxiella burnetii during its biphasic developmental cycle revealed by cryo-electron tomography
| العنوان: | Morphological remodeling of Coxiella burnetii during its biphasic developmental cycle revealed by cryo-electron tomography |
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| المؤلفون: | Shepherd, Doulin C., Kaplan, Mohammed, Vankadari, Naveen, Kim, Ki Woo, Larson, Charles L., Dutka, PrzemysÅ‚aw, Beare, Paul A., Krzymowski, Edward, Heinzen, Robert A., Jensen, Grant J., Ghosal, Debnath |
| المصدر: | iScience, 26(7), 107210, (2023-07-21) |
| بيانات النشر: | Cell Press |
| سنة النشر: | 2023 |
| المجموعة: | Caltech Authors (California Institute of Technology) |
| مصطلحات موضوعية: | Multidisciplinary |
| الوصف: | Coxiella burnetii is an obligate zoonotic bacterium that targets macrophages causing a disease called Q fever. It has a biphasic developmental life cycle where the extracellular and metabolically inactive small cell variant (SCV) transforms inside the host into the vegetative large cell variant (LCV). However, details about the morphological and structural changes of this transition are still lacking. Here, we used cryo-electron tomography to image both SCV and LCV variants grown either under axenic conditions or purified directly from host cells. We show that SCVs are characterized by equidistant stacks of inner membrane that presumably facilitate the transition to LCV, a transition coupled with the expression of the Dot/Icm type IVB secretion system (T4BSS). A class of T4BSS particles were associated with extracellular densities possibly involved in host infection. Also, SCVs contained spherical multilayered membrane structures of different sizes and locations suggesting no connection to sporulation as once assumed. ; © 2023 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0Test/). ; This project was funded by the National Institutes of Health (grant R01 AI127401 to G.J.J.), an NHMRC grant (APP1196924 to D.G.), and the Intramural Research Program of the National Institutes of Health, National Institute of Allergy and Infectious Diseases (Grant number AI000931-20 to R.A.H. and C.L.L.). D.C.S. is supported by the Melbourne Research Scholarship. We are grateful to Prof. Elitza Tocheva for insightful discussions, and Somavally Dalvi for her help with figure preparation. ; Conceptualization, D.G., G.J.J., and M.K.; Methodology, D.G., M.K., D.C.S., C.L.L., P.D., P.A.B., and E.K.; Investigation, M.K., D.G., D.C.S., C.L.L., P.D., and P.A.B.; Formal Analysis, M.K., D.G., D.C.S., C.L.L., P.D., P.A.B., K.W.K., N.V., E.K., and R.A.H.; Writing– Original Draft, D.G., M.K., and N.V.; Writing– Review and Editing, M.K., D.G., D.C.S., C.L.L., P.D., ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://ars.els-cdn.com/content/image/1-s2.0-S2589004223012877-mmc1.pdfTest; https://doi.org/10.1016/j.isci.2023.107210Test; oai:authors.library.caltech.edu:w9khr-5r449; https://www.ncbi.nlm.nih.gov/pmc/PMC10362272Test |
| DOI: | 10.1016/j.isci.2023.107210 |
| الإتاحة: | https://doi.org/10.1016/j.isci.2023.107210Test https://www.ncbi.nlm.nih.gov/pmc/PMC10362272Test |
| حقوق: | info:eu-repo/semantics/openAccess ; Creative Commons Attribution Non Commercial No Derivatives 4.0 International ; https://creativecommons.org/licenses/by-nc-nd/4.0/legalcodeTest |
| رقم الانضمام: | edsbas.F885A2D3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.isci.2023.107210 |
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