دورية أكاديمية
A high-resolution melt curve toolkit to identify lineage-defining SARS-CoV-2 mutations
| العنوان: | A high-resolution melt curve toolkit to identify lineage-defining SARS-CoV-2 mutations |
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| المؤلفون: | Fraser, Alice J., Greenland-Bews, Caitlin, Kelly, Daniel, Williams, Christopher T., Body, Rick, Adams, Emily R., Cubas Atienzar, Ana, Edwards, Thomas, Allen, David J., Dark, Paul |
| المصدر: | Fraser , A J , Greenland-Bews , C , Kelly , D , Williams , C T , CONDOR Steering Group , LSTM Diagnostics Group , Body , R , Adams , E R , Cubas Atienzar , A , Edwards , T , Allen , D J & Dark , P 2023 , ' A high-resolution melt curve toolkit to identify lineage-defining SARS-CoV-2 mutations ' , Scientific Reports , vol. 13 , no. 1 , 3887 . https://doi.org/10.1038/s41598-023-30754-1Test |
| سنة النشر: | 2023 |
| المجموعة: | The University of Manchester: Research Explorer - Publications |
| مصطلحات موضوعية: | Humans, COVID-19, SARS-CoV-2/genetics, Mutation, Biological Assay, Genomics |
| الوصف: | The emergence of severe acute respiratory syndrome 2 (SARS-CoV-2) variants of concern (VOCs), with mutations linked to increased transmissibility, vaccine escape and virulence, has necessitated the widespread genomic surveillance of SARS-CoV-2. This has placed a strain on global sequencing capacity, especially in areas lacking the resources for large scale sequencing activities. Here we have developed three separate multiplex high-resolution melting assays to enable the identification of Alpha, Beta, Delta and Omicron VOCs. The assays were evaluated against whole genome sequencing on upper-respiratory swab samples collected during the Alpha, Delta and Omicron [BA.1] waves of the UK pandemic. The sensitivities of the eight individual primer sets were all 100%, and specificity ranged from 94.6 to 100%. The multiplex HRM assays have potential as a tool for high throughput surveillance of SARS-CoV-2 VOCs, particularly in areas with limited genomics facilities. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41598-023-30754-1 |
| الإتاحة: | https://doi.org/10.1038/s41598-023-30754-1Test https://research.manchester.ac.uk/en/publications/cb2a3e5b-e457-4982-825a-92489ca3016eTest http://www.scopus.com/inward/record.url?scp=85150228906&partnerID=8YFLogxKTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.F87BC884 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-023-30754-1 |
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